作　　者： ; ; ; ;

机构地区： 湖南大学土木工程学院

出　　处： 《环境工程学报》 2013年第7期2711-2715,共5页

摘　　要： 利用水解溶菌酶对SBR系统中的剩余污泥进行减量。通过与未加水解溶菌酶的相同系统对比,研究了水解溶菌酶作用下的SBR系统中剩余污泥的减量效果与微生物群落结构的变化。结果表明,在50 d的运行期内,水解溶菌酶作用下的SBR系统中剩余污泥减量总计达到76.3%,同时该系统对COD与TN的平均去除率分别为88.2%与53.8%。通过PCR-DGGE分析可知,随着运行时间的增加两系统微生物群落结构的差异逐步明显,SBR系统中原有的部分优势微生物在水解溶菌酶的作用下逐渐减弱。另外,对微生物群落的部分优势细菌进行克隆测序和系统发育树分析,通过鉴定获得的7条细菌的16S rDNA序列,它们分别与放线菌和杆菌同源性在97%以上。 The lysozyme was employed to reduce the excess sludge in the SBR system. The effects of the lysozyme on sludge reduction and microbial community structure in the SBR system were evaluated through the comparative observation of the sludge in a reference SBR without lysozyme addition. Experimental results showed that during 50 days operation the lysozyme could effectively reduce the production of excess sludge by 76.3% in the test SBR system. The average removal efficiencies of chemical oxygen demand and total nitrogen in the SBR with lysozyme were 88.2% and 53.8%, respectively. The PCR-DGGE analysis showed that in the two systems the differences of the microbial community structure were formed gradually, and the part of dominant bacteria was weakened gradually in the SBR with lysozyme addition. Part of the microbial community of dominant bacteria was analyzed through cloning sequencing and phylogenetic analysis. Ten bacterial 16S rDNA sequences were identified. The homology of these bacteria was more than 97% compared with Actionbacterium and B. anthracis.

关 键 词： 污泥减量 水解溶菌酶

领　　域： [环境科学与工程]