机构地区: 吉林农业大学农学院
出 处: 《大豆科学》 2013年第3期420-424,共5页
摘 要: 高密度分子遗传连锁图谱在大豆重要性状基因定位、精确图位基因克隆、比较基因组研究以及分子标记辅助育种等方面具有极其重要的应用价值。目前,大豆分子遗传连锁图谱日趋饱和,分子标记类型主要有限制性片段长度多态性(RFLP)、随机扩增多态性(RAPD)、简单序列重复(SSR)、扩增片段长度多态性(AFLP)、序列标签位点(STS)、表达序列标签(EST)和单核苷酸多态性(SNP)等;作图群体主要有F2群体、重组自交系(RIL)和双单倍体(DH)等。简要论述了大豆遗传连锁图谱的研究现状并展望了其发展趋势,以期促进大豆遗传连锁图谱的发展及在大豆育种工作中的应用。 High - density molecular genetic linkage map is extremely important in studies of QTL, accurate map - based clo- ning, comparative genomics and molecular assisted selection in soybean. At present, several soybean genetic linkage maps have been constructed and integrated. The main molecular makers include restriction fragment length polymorphism ( RFLP), random amplified polymorphic DNA ( RAPD ), simple sequence repeat ( SSR ), amplified fragment length polymorphism (AFLP) , sequence tagged site (STS) , expressed sequence tag (EST) and single-nucleotide polymorphism (SNP) , etc. The mapping population involve F2, recombinant inbred line (RIL) and double haploid( DH), etc. The research status and future trends of soybean genetic linkage map were briefly discussed to promote the application of the soybean genetic linkage map in soybean breeding.