作　　者： ; ; ; ; ; ;

机构地区： 中国科学院南海海洋研究所海洋生物资源可持续利用重点实验室

出　　处： 《科学通报》 2013年第17期1590-1595,共6页

摘　　要： 造礁石珊瑚为珊瑚礁生态系统中的框架生物,研究其重要功能基因对于理解造礁石珊瑚对环境变化的响应有重要指示意义.本研究提取了澄黄滨珊瑚(Porites lutea)的总RNA,通过RT-RCR得到cDNA,并以cDNA为模板设计引物进行test PCR,获得大小为254bp的ferritin基因cDNA序列.分析表明,该部分序列编码了84个氨基酸,其中包含一个亚铁氧化酶活性功能区,而该活性功能区又有一个铁离子通道,其与多孔鹿角珊瑚(Acropora millepora)的ferritin基因相似度较高,达85%,覆盖度为100%,推测该基因序列在种内和种间都较保守,已向GenBank提交序列,并获得登录号为JQ305796.该序列可用于实时定量PCR技术(qRT-PCR)及cDNA末端快速扩增技术(RACE)等后续分子生物学研究. Coral reef, the main component of the coral reef ecosystem, is extremely sensitive to environmental changes. Research on the functional genes of coral is very valuable. We extracted total RNA from the coral Porites lutea and reversely transcribed it into cDNA. The cDNA was used as a template to amplify part of theferritin gene by PCR. We obtained a clean 254-bp band, and sequenced. The sequence encodes 83 amino acids of the ferritin protein, containing a ferroxidase di-iron center and an iron ion channel. Similarity searching by BLAST identified 100% coverage with ferritin in the coral Acropora millepora and 85% similarity, suggesting that this gene is highly conserved across species. The sequence can be accessed from the GenBank database with the accession number JQ305796. This sequence is a useful gene for future molecular biological studies such as qRT-PCR and RACE.

关 键 词： 造礁石珊瑚

领　　域： [生物学]