机构地区: 广州中医药大学中药学院
出 处: 《中山大学学报(自然科学版)》 2013年第3期77-82,共6页
摘 要: 在研究珊瑚微生物混合共培养时发现:在混合培养中南海珊瑚内生细菌L-4对真菌的次生代谢产物明显产生影响,甚至产生此二微生物单独培养时所不产生的物质。对L-4发酵液进行次生代谢产物研究,从中分离纯化得到8个单体化合物,通过现代波谱分析(MS、NMR等)和物理常数对照等手段确定其结构依次为:环(色-丙)二肽(1)、环(甘-酪)二肽(2)、环(丙-酪)二肽(3)、环(缬-酪)二肽(4)、环(甘-苯丙)二肽(5)、环(甘-脯)二肽(6)、环(丙-缬)二肽(7)、环(甘-丙)二肽(8)均为环二肽类物质,且均首次从南海珊瑚内生细菌中分离得到,对进一步研究其可能的代谢机制具有重要意义。 The influence of secondary metabolites on the mix-cultivate marine bacteria and fungus were studied. The result indicated that bacteria obviously affected the secondary metabolites of fungus in the mix-cultivate, and even produce substances that were not found in the fungus or bacteria separatel ture by TLC. Eight compounds were obtained from the fermentation broth of coral-derived bacteria y cul- L-4 in South China Sea. Their structures were determined to be cyclo (Trp-Ala) (1), cyclo (Gly-Tyr) (2), cyclo (Ala-Tyr) (3), and cyclo (Val-Tyr) (4) cyclo (Gly-Phe) (5), cyclo (Gly-Pro) (6), cyclo (Ala-Val) (7), and cyclo (Gly-Ala) (8) by spectroscopic analysis. The compounds 1, 2, 3, 4, 5, fi, 7, 8 were all cyclo-dipeptides and first obtained from the marine coral-derived bacterium of South China Sea, which attributed to further research metabolism of mix-cultivation.