作　　者： ; ; ; ; ; ; ; ; ; ;

机构地区： 广西水产研究所

出　　处： 《水产学报》 2013年第6期927-935,共9页

摘　　要： 为获知近年广西罗非鱼链球菌病流行菌种及其基因型变化信息,采用特异PCR方法对2006—2012年从广西发病罗非鱼分离获得的77株临床菌株进行鉴定,并通过脉冲电场凝胶电泳(PFGE)对2006—2011年分离获得的37株流行菌株进行基因型分析。结果显示,其中20株鉴定为海豚链球菌其余57株鉴定为无乳链球菌。2006—2007年获得的19株流行菌株中有18株为海豚链球菌(94.7%),仅1株无乳链球菌;2009—2012年分离的58株流行菌株中56株为无乳链球菌(96.6%),仅2株海豚链球菌。PFGE图谱聚类显示,海豚和无乳链球菌分别聚类为两个大分支,20株海豚链球菌共产生4种PFGE带型,带型相似度为83.9%～100%;17株无乳链球菌共产生5种PFGE带型,带型相似度为47.4%～100%。研究表明,广西罗非鱼链球菌病流行菌种已从过去(2008年前)以海豚链球菌为主转变为现在(2009—2012年)以无乳链球菌为主;流行菌株PFGE基因型存在多样性。 To acquire the data upon the clinical dominant strains of tilapia Streptococcus spp.and their genotype variations in Guangxi province,a total of 77 clinical Streptococcus spp.strains collected from the epidemic areas of Guangxi from 2006 to 2012 were identified by species-specific PCR.Among them,37 strains collected from 2006 to 2011 were genotyped by the pulse field gel electrophoresis（PFGE）method.As a result,57 out of 77 strains were identified as Streptococcus agalactiae,and the rest were determined as S.iniae by PCR identification process.As is shown,the dominant strains from 2006 to 2007 were S.iniae,accounted for 94.7%（18/19）,only 5.3%（1/19）was S.agalactiae strains.Between 2009 and 2012,the dominant strains,however,became S.agalactiae,which accounted for 96.6%（56/58）,only 3.4%（2/58）was referred as S.iniae strains.The strains were divided into two branches by PFGE genotyping.20 S.iniae strains were in one classification with 4 PFGE genotypes,the similarities of which were 83.9%-100%.17 S.agalactiae strains were put in another branch with 5 PFGE genotypes,the similarities of which were between 47.4% and 100%.Our data confirmed that the prevalent strains of tilapia Streptococcus spp.in Guangxi province have been shifting from S.iniae.

关 键 词： 罗非鱼 无乳链球菌 海豚链球菌 脉冲场凝胶电泳 基因型

领　　域： [农业科学] [农业科学]