作　　者： ; ; ; ; ; ; ;

机构地区： 贵州大学

出　　处： 《生物技术通报》 2013年第5期86-92,共7页

摘　　要： 旨在克隆牛FATP1基因5'侧翼启动子,研究其特异性转录调控元件的调控机制。利用PCR方法扩增牛FATP1基因5'侧翼区,并通过产物纯化、连接、转化及测序比对,获得2 164 bp(-1 969-+194 bp)的5'侧翼启动子序列。利用生物信息软件对获得的2 164 bp 5'侧翼启动子克隆载体进行分析预测,发现其有4处转录起始点和40个潜在转录因子结合位点;该序列与人、小鼠、大鼠和狗的同源性分别为74.1%、71.1%、72.0%、62.8%,且不同物种FATP1基因5'侧翼区的同源序列主要集中在转录起始点上游-578--93 bp区域,保守性较强的区域在转录起始点上游-263--174 bp,推测转录起始点上游-263--174 bp可能是其核心启动子区域,从而成功克隆了FATP15'侧翼启动子序列2 164 bp,初步预测了该基因的核心启动子区域。 The experiment was conducted to clone 5＇-flanking region of bovine FATP1 gene to study regulatory mechanism of the specific transcriptional regulation region.The 5＇-flanking region of bovine FATP1 gene was amplified with PCR,and then the 2 164 bp（-1969-＋194 bp）5＇-flanking promoter sequence was obtained by product purification,ligation,transformation,and sequence comparison.The obtained cloning vector of 5＇-flanking region of bovine FATP1 gene was analyzed by the promoter software in this study.The results indicated that there were 4 transcription start sites and 40 potential transcription factor binding sites.Comparing bovine with human,mouse,rat and dog,the homology of 5＇-flanking promoter sequence were 74.1%,71.1%,72.0%,and 62.8%,respectively.The conservative region was mainly concentrated in-578--93 bp of the upstream of transcription start sites,and the highly conserved region was in-265--162 bp of the upstream of transcription start sites,which may be conclude that the-265--162 bp of the upstream of transcription start sites was the core promoter region of the gene.The 2 164 bp 5＇-flanking region of the bovine FATP1 gene was cloned successfully and the core promoter region was preliminary predicted in the gene.

关 键 词： 牛 克隆 基因 启动子 转录因子

领　　域： [生物学]