作　　者： ; ; ; ; ;

机构地区： 福建省水产研究所

出　　处： 《海洋科学》 2013年第3期38-44,共7页

摘　　要： 对赤点石斑鱼(Epinephelus akaara)精子的激活特点、超低温冷冻方案筛选、短期保存、受精实验等方面进行了研究。结果表明:水温25℃、比重为1.018的海水对赤点石斑精子具有最佳的激活效果,活力为(82.50±4.18)%;在进行超低温冷冻时,选择距离液氮面7 cm的高度进行降温,获得冻后活力为(65.83±3.76)%,显著高于1、3、15 cm的处理组(P<0.0001),利用冻存液B(含30 g/L海藻糖、10%DMSO的生理盐水)冻存赤点石斑精子,冻精活力为(67.92±3.96)%,显著高于另外两种冻存液(P<0.05);通过优化的方案(冻存液B,7 cm高度)冻存得到精子可获得较好的受精效果,受精率为(74.55±4.31)%,而相应的鲜精受精率可达(87.42±4.63)%,二者间的差异具有显著性(P=0.017);短期保存的结果表明,赤点石斑鱼精子在4℃环境中保存约两周后活力降低为0。 In this paper, the activation characteristics, cryopreservation program optimization, short-term preservation and fertilization of red spotted grouper sperm were studied in detail. The results showed that seawater with specific gravity of 1.018 and temperature of 25℃ has the best activating effect and the sperm motility was （82.50±＋4.18）%. Frozen samples with 7 cm height above the liquid nitrogen surface reached a post-thaw motility of （65.83±3.76）%, which was significantly higher than other samples （P〈0.0001）. Using cryopreservation solution B （containing 30 g/L trehalose, 10% DMSO and saline）, the post-thaw motility was （67.92±3.96）%, which is significantly higher than those using the other two cryopreservation solutions. Samples frozen with optimized program （cryopreservation solution B, height of 7 cm） could achieve good fertilization rate （74.55±4.31）%, which was significantly lower than that of the corresponding fresh sperm （87.42±4.63）%, P〈0.05）. After two week＇s preservation at 4 ℃, the red spotted grouper sperm motility reduced to 0.

关 键 词： 赤点石斑鱼 精子 超低温冷冻 低温保存

领　　域： [农业科学]