作　　者： ; ; ; ;

机构地区： 广州医学院

出　　处： 《中国生物工程杂志》 2013年第4期92-100,共9页

摘　　要： 目的:建立一种能同时检测幽门螺杆菌克拉霉素(23S)、喹诺酮(gyrA)耐药位点和人PPI代谢相关CYP2C19的基因多态性情况的基因芯片检测方法。方法:通过分析筛选人基因组CYP4502C19*2,CYP4502C19*3及幽门螺杆菌克拉霉素和喹诺酮类耐药位点的DNA序列,设计制备了HP感染个体化药物治疗检测基因芯片,并对其特异性、灵敏度、重复性进行了评价。结果:该芯片可以检测出不低于103CFU/ml的幽门螺杆菌和不低于2ng/μl的人基因组DNA。196例临床标本的芯片检测结果与测序结果基本一致。结论:应用可视化基因芯片进行幽门螺杆菌感染个体化药物治疗相关基因检测具有较高的特异性和敏感性,结果准确且易判读,具有较好的应用前景,可以为临床医生提供用药指导。 Objective： To establish a method of visualized gene chip detection that can simultaneously measure the gene polymorphism of CYP2C19 that is related to clarithromycin and quinolones resistance mutation in Helicobacter pylori infection and to the metabolism of proton pump inhibitor. Method： Through analysis the sequence of human CYP2C19 ＊ 2 , CYP2C19 ＊ 3 and the targets related to resistance to clarithromycin and quinolone, a gene chip to test the resistence of Helicobacter Pylori was established. The specificity, sensitivity and repeatability of the gene chip was evaluated in the optimized PCR system, with hybridization reaction and visualized detection. Result： The results of detection of 196 clinical samples using gene chips were consistent with that by sequencing. The detection sensitivity of the chip for Hp was 10^3CFU/ml, and that for human genomie DNA was 2ng/μl. Conclusion： This method was quick, highly specific, sensitive, and economic. With its clinical application potential, it is promising in providing medication guidance for Hp elimination in clinical use and individualized treatment.

关 键 词： 幽门螺杆菌 耐药 多态性 个体化治疗 基因芯片 可视化检测法

领　　域： [生物学]