作　　者： ; ; ; ;

机构地区： 广东省疾病预防控制中心

出　　处： 《中国卫生检验杂志》 2013年第3期579-581,共3页

摘　　要： 目的:建立一种快速、准确测定黄酒中氨基甲酸乙酯(EC)含量的气相色谱-质谱(GC/MS)法。方法:酒样添加d5-EC同位素内标,经硅藻土层析柱吸附,乙醚洗脱,用GC/MS测定,内标法定量。色谱柱为HP-INNOWAX石英毛细管柱(30 m×0.25 mm×0.25μm)。结果:进行高、中、低三个浓度水平的加标回收试验,平均回收率为89.2%～102%,RSD为2.2%～4.7%(n=6)。应用本方法检测了55份广东本地生产的黄酒产品,检出率为100%,含量范围10μg/kg～1200μg/kg。结论:该方法快速、准确、分析成本低,能满足大批量酒类样品中氨基甲酸乙酯快速分析的要求。 Objective： A rapid method has been established for the determination of ethyl carbamate （EC） residue in yellow wine. To survey the levels of EC in yellow wine sold in Guangdong province. Methods： The sample was added with ethyl carbamate- d5 （D5 -EC） , then absorbed onto a kieselguhr column and eluted with ethyl ether, and separated by gas chromatography on a HP- INNOWAX capillary column（30 m ×0.25 mm×0.25μm） , identified and quantified by mass spectrometry in SIM mode. Results： The mean recoveries ranged from 89.2% to 102% with the relative standard deviation of 2.2% to 4.7% （n = 6）. A total of 55 samples were detected by the method. The detection rate of EC was 100% at levels between 10 μg/kg - 1200 μg/kg. Conclusion： The results showed that the method was suitable for the fast analysis of EC residue in yellow wine.

关 键 词： 氨基甲酸乙酯 气质联用 同位素稀释 黄酒

领　　域： [理学] [理学]