作　　者： ; ;

机构地区： 暨南大学生命科学技术学院生物工程学系

出　　处： 《生物技术》 2000年第5期1-3,共3页

摘　　要： 将携带有T7噬菌体RNA聚合酶基因的质粒和neo抗性基因质粒共转化CHO细胞 ,经狭缝RNA和Southernblot杂交实验证实获得表达T7噬菌体RNA聚合酶基因的细胞株。pT7CAT质粒转染未表达和表达的细胞株 ,实验显示两者都能表达报道基因 ,但效率不同。实验构建了不同表达水平的T7启动子表达系统。 The plasmid carrying the gene for T7 RNA polymerase and plasmid carrying neo r gene were cotransfected into CHO cells.Slot-blot and Southern tests showed the T7 RNA polymerase gene was integrated into host chromosome and expressed in cells.Plasmid pT7CATcarrying report gene was transfected into CHO cells with and without T7 polymerase gene,respectively.The results demonstrated that CAT report genes were expressed in different level in host cells with and without integrated T7 polymerase gent.

关 键 词： 启动子 真核生物表达系统 聚合酶

领　　域： [生物学]