作　　者： ; ; ; ; ;

机构地区： 南京农业大学

出　　处： 《土壤学报》 2013年第2期381-387,共7页

摘　　要： 利用实时荧光定量PCR方法对田间条件下连作番茄和辣椒施用生物有机肥(BOF)和常规施肥(CK)的根际土壤微生物中青枯病原菌和功能菌群(固氮菌和荧光假单胞菌)的数量进行定量研究。结果表明:与CK相比,BOF处理的番茄和辣椒产量分别提高了26.0%和19.9%,青枯病发病率分别降低了41.5%和44.7%,番茄和辣椒植株根际土壤固氮菌数量分别增加了23.5%和25.8%、荧光假单胞菌数量分别增加了29.5%和20.2%、病原菌数量分别减少了73.2%和90.1%。生物有机肥能够调控根际微生物区系的组成,降低土传病害的发病率,促进作物健康生长;实时荧光定量PCR方法能够快速准确地检测根际土壤中功能微生物种群数量变化。 Populations of pathogenic Ralstonia solanacearum and functional bacteria groups in the rhizospheric soils of continuous cropping tomato and pepper applied with a kind of bio-organic fertilizer （BOF） and with conventional fertil- izer （CK） were studied using the real-time quantitative PCR techniques. As compared with CK, BOF increased the yields of tomato and pepper by 26.0% and 19.9% , respectively, and decreased the incidence of bacterial wilt on tomato and pepper by 41.5% and 44.7% , respectively. BOF increased the population of Azotobacter and Pseudomonasflurosecens by 23.5% and 29.5% , and by 25.8% and 20.2% , and decreased the population of R. solanacearum by 73.2% and by 90.1% , respectively, in the rhizospheric soil of healthy tomato and pepper. In conclusion, the bio-organic fertilizer is ca- pable of regulating the composition of microflora in rhizosphere soil of crops, reducing the incidence of soil-borne plant disease, and promoting plant growth. Real time PCR techniques are quick and accurate in determination of the population of functional microbes in soil.

关 键 词： 荧光定量 生物有机肥 根际土壤 青枯病 固氮菌 荧光假单胞菌

领　　域： [生物学]