机构地区: 华南农业大学资源环境学院昆虫学系
出 处: 《环境昆虫学报》 2013年第1期118-121,共4页
摘 要: 沃尔巴克氏体(Wolbachia)是广泛分布在节肢动物生殖组织中的一类细胞质遗传的细菌,它们可以调控宿主的生殖活动。利用沃尔巴克氏体wsp基因的1对通用引物(81F,691R)从新入侵我国的扶桑绵粉蚧Phenacoccus solenopsis Tinsley雌成虫的总DNA中扩增到1个550bp左右的片段,并克隆得到了Wolbachia的wsp基因序列,首次发现了沃尔巴克氏体对扶桑绵粉蚧的感染现象。 Wolbachia is a tissues of arthropods. The type of cytoplasmically inherited bacteria and widely found in reproductive bacteria may spread in host populations and regulate host reproduction. In this study, PCR was used to determine the presence of Wolbachia by using general wsp primers (81F, 691 R), and 550 bp wsp sequence of Wolbachia has been revealed. Wolbachia infection of the female of Phenacoccu~; solenopsis Tinsley has been detected for the first time.