作　　者： ; ; ; ; ; ; ; ;

机构地区： 华南农业大学

出　　处： 《畜牧兽医学报》 2013年第3期427-433,共7页

摘　　要： 高致病性禽流感严重制约着禽类产业链的健康发展,以H5N1为代表的高致病性禽流感病毒能够跨越种间障碍感染人,对公共卫生安全造成极大危害。而预防禽流感的传统疫苗主要是全病毒灭活苗,该类疫苗使用量大,灭活不全时易造成散毒,并给流行病学监测带来困难。研发更加安全、高效的新型疫苗势在必行。本研究选取H5N1亚型流感病毒的HA蛋白作为靶抗原,构建重组杆状病毒BV-G-H5N1-HA。重组病毒本身可刺激机体天然免疫反应,通过基因改造一方面能在真核细胞内大量表达靶抗原,同时可以将靶抗原展示在重组杆状病毒囊膜表面,兼具DNA疫苗和亚单位疫苗的安全性和有效性。小鼠试验结果表明,BV-G-H5N1-HA免疫组能产生较高水平的中和抗体、HI抗体,BV-G-H5N1-HA免疫组攻毒保护率达91.7%。以上数据表明,BV-G-H5N1-HA可以作为一种新型候选疫苗,为H5N1亚型高致病性禽流感的防控贡献力量,为其他亚型疫苗的研究提供参考。 Avian influenza is one of the deadly infectious diseases of poultry, which influences the development of agricultural economy of China greatly. Highly pathogenic avian influenza （HPAI） virus like HSN1 can even break the species barrier and infect human, which may pose great threat to public health. Currently, inactivated vaccines are the main vaccine in preventing avian influen- za, which may accelerate virus transmission if we can＇t get the virus completely inactivated. Therefore, we need safer and more effective vaccines. In this study, we constructed a recombi- nant baculovirus BV-G-HSN1-HA, expressing HA protein in mammalian cell and exhibiting HA protein on the surface of the viral envelope simultaneously. Animal experiment was conducted by vaccinating mice intramuscularly with BV-G-H5N1-HA, pc-HSN1-HA, AcMNPV-WT （wildtype AcMNPV） and PBS, respectively, then the mice were challenged intranasally （i. n. ） with A/Chicken/Guangzhou/M/2008（HSN1）. Results showed that BV-G-HSNll-HA can induce higher concentrations of neutralizing antibody and HI antibody in the immune group, and provide a considerable protection rate of 91.7Y0. All these data indicate that BV-G-H5N1-HA may be a novel vaccine candidate which helps to prevent and control of HPAI in the future. Moreover, the application of novel baculovirus vector will provide important insights into the field of animal vaccine development.

关 键 词： 禽流感 疫苗 杆状病毒表达系统

领　　域： [农业科学] [农业科学] [农业科学]