作　　者： ; ; ; ; ;

机构地区： 沈阳农业大学生物科学技术学院

出　　处： 《中国油料作物学报》 2013年第1期58-63,共6页

摘　　要： 从北部湾地区淡水水域分离得到一株高含油量小球藻藻株,将其培养在高碳培养基TAP上,研究不同浓度IAA和ABA对其生物量和油脂积累的影响。结果表明:添加10μmol/L IAA对藻株的生长有明显的促进作用,其最大生物量比对照组增加了58.24%,但其油脂含量却比对照组降低了34.71%;而当增加IAA浓度为20μmol/L时,对藻株的生长表现出明显的抑制作用,其最大生物量比对照组减少了53.60%,但其油脂含量与对照组相比显著增加,增加了95.46%。添加2μmol/L ABA对藻株生长的抑制作用极其明显,其生物量比对照组降低了57.52%,但其油脂含量与对照组相比显著提高,提高了121.49%。同时添加IAA(20μmol/L)和ABA(2μmol/L)的实验藻种的蛋白质和总糖的含量较对照组有所降低,此外也使叶绿素含量和光合效率降低。 A high oil microalgae strain collected from fresh waters of Beibu Bay rella sp. and cultured in high - carbon medium TAP. Effects of IAA and ABA on its area was indentified as Chlobiomass and oil accumulation were studied. The results showed that 101μmol/L IAA promoted the growth of algae strains significantly. The max biomass increased by 58, 24% compared to control while oil content decreased by 34.71%. In contrast,20μmol/L IAA clearly inhibited the growth of algae strains and the decrease in max biomass was 53.60%. However, oil con- tent increased by 95.46%. The inhibition of ABA on the growth of algae strains was evident. There was a 57.52% decrease in its biomass compared to control group and a 121.49% increase in its oil content. The protein, total sugar content, chlorophyll content and photosynthetic efficiency of the algae were decreased compared to control when IAA（20μmol/L） and ABA （2μmol/L） were added to the medium.

关 键 词： 小球藻 植物激素 细胞生长 油脂含量 光合效率

领　　域： [生物学]