作　　者： ; ;

机构地区： 暨南大学

出　　处： 《暨南大学学报（自然科学与医学版）》 2000年第5期95-100,共6页

摘　　要： 目的 :分析花生球蛋白、伴花生球蛋白Ⅰ和伴花生球蛋白Ⅱ及其组成多肽的氨基酸组成 ,筛选高甲硫氨酸多肽并研究其在花生种子发育和萌发过程中的合成降解规律 方法 :花生球蛋白、伴花生球蛋白Ⅰ和Ⅱ经SephadexG - 10 0纯化后 ,再以制备电泳和电泳洗脱得到高纯度的 5种多肽 ;酸水解法测定了花生球蛋白、伴花生球蛋白Ⅰ和Ⅱ以及 5种多肽的氨基酸组成 ;WesternBlot分析了种子发育和萌发过程中 17 5× 10 3多肽的合成降解规律 结果 :花生球蛋白、伴花生球蛋白Ⅰ和Ⅱ以及 5种多肽均含有 17种氨基酸 (包括 8种必需氨基酸 ) ,其中天冬氨酸、谷氨酸和精氨酸含量为最高 ,约为总量的 4 5% ;而甲硫氨酸含量水平都极低 在这 5种多肽中 ,17 5× 10 3多肽的甲硫氨酸含量最高 17 5× 10 3多肽在花生种子发育早期开始合成 ,而在种子吸涨 60h时发生降解 结论 :17 5× 10 3多肽的甲硫氨酸含量最高 。 Aim: To study the amino acid-composition of purified 3 major components(arachin, conarachin I and conarachin II) and the five purified polypeptides. To separate the methionine-rich proteins and analyze their changes of synthesis and degradation during development and germination in peanut seeds. Methods: The 3 major components of salt-soluble proteins were purified by Sephadex G-100 gel filtration. Five purified polypeptides were harvested by SDS-PAGE. Amino acid-composition of the 3 purified major components and the five polypeptides were analyzed. The rules for synthesis and degradation of 17 5×10 3 polypeptide during the development and germination of seeds had been investigated by Western Blot. Results: 17 amino acids, included 8 essential amino acids, were detected in all 3 major components and the five purified polypeptides. Asparatic acid, glutamic acid and arginine were most predominant, methionine was extremely low in contrast, chiefly existing in 17 5×10 3 polypeptide of the five polypeptides. In the development, the synthesis of 17 5×10 3 polypeptide was almost undetectable at first. Afterwards, the accumulation was significant. It began to degraded considerly in 60 hours and disappeared completely soon. Conclusion: 17 5×10 3 polypeptide had higher methionine content. The synthesis and degradation patterns of 17 5×10 3 polypeptide were rather different from those of arachin and conarachin I. [

关 键 词： 花生种子 氨基酸 多肽 合成降解

领　　域： [生物学] [农业科学]