作　　者： ; ; ; ; ;

机构地区： 华南理工大学生物科学与工程学院广东省发酵与酶工程重点实验室

出　　处： 《中国酿造》 2013年第2期9-12,共4页

摘　　要： 毕赤酵母表达系统是近年发展较为突出的表达系统之一。大量研究表明,低温诱导在毕赤酵母发酵过程中对促进细胞物质及能量代谢,提高胞内醇氧化酶活力及能量水平,降低细胞死亡率及蛋白酶作用有明显影响;此外,外源蛋白表达过程中的聚集与降解作用也与诱导温度有关。该文从诱导温度角度对重组毕赤酵母表达外源蛋白的研究进展进行了综述。 Pichia pastoris expression system is one of the most prominent expression systems which is widely applied and developed in recent years. A large number of researches indicated that low inducing temperature plays an important role in promoting cell material and energy metabolism, im- proving intracellular alcohol oxidase （AOX）, energy level, as well as reducing the cell mortality rate and affecting proteases during fermentation pro- cess of P. pastoris. In addition, degradation and aggregation of foreign protein are also related to the temperature during its expression phase. This pa- per reviewed the research progress of temperature induction on the expression of foreign proteins in P. pastoris.

关 键 词： 毕赤酵母 诱导温度 物质及能量代谢

领　　域： [生物学]