作　　者： ; ; ; ;

机构地区： 南京农业大学动物科技学院

出　　处： 《江苏农业学报》 2013年第1期140-146,共7页

摘　　要： 为评价甲酸与纤维素酶和木聚糖酶处理后多花黑麦草与白三叶混合青贮料发酵品质的变化,试验将混合青贮料分为对照组(未处理)、甲酸添加组、纤维素酶添加组、甲酸+纤维素酶添加组、木聚糖酶添加组、甲酸+木聚糖酶添加组、纤维素酶+木聚糖酶添加组、甲酸+纤维素酶+木聚糖酶添加组共8组,每组3个重复。青贮原料按80%多花黑麦草与20%白三叶配制。在青贮第0 d、第2 d、第4 d、第6 d、第8 d和第30 d分别对青贮料进行化学分析。结果显示:在青贮结束时,所有处理组青贮料pH值显著低于对照组(P<0.05);在青贮过程中,甲酸、酶或甲酸+酶处理组显著增加青贮料中水溶性碳水化合物含量(P<0.05);酶处理组乳酸含量和乙酸含量分别高于含甲酸处理组和对照组(P<0.05);所有处理组NH3-N含量显著低于对照组(P<0.05),含甲酸处理组NH3-N含量显著低于酶处理组(P<0.05);酶处理组显著降低了青贮料中中性洗涤纤维与酸性洗涤纤维含量(P<0.05)。表明,添加甲酸、酶制剂能改善多花黑麦草与白三叶混合青贮的质量,其中酶处理比甲酸处理效果更好。 To evaluate the effects of formic acid, cellulase and xylanase on fermentation quality of mixture silage during ensiling, 2 000 g chopped Lolium multiflorum（ 80% ） and Trifolium repens（ 20% ） mixtures were ensiled in laboratory plastic bag either untreated （control） or treated with formic acid, cellulase, formic acid plus cellulase, xylanase, formic acid plus xylanase, cellulase plus xylanase and formic acid plus cellulase plus xylanase, respectively. Triplicate bags were opened at 0 d, 2 d, 4 d, 6 d, 8 d and 30 d of ensiling for chemical analyses. The pH value in all treated silages was lower （ P〈0. 05 ） than control at the end of ensiling. The formic acid, enzyme or formic acid plus enzyme treatments enhanced （P〈0. 05 ） water soluble carbohydrate content significantly compared with control at all ensiling periods. The lactic acid content and acetic acid content were higher （P〈O. 05 ） in the enzyme treatment than those in the formic acid-contained treatments and control, respectively. However, the acetic acid content was lower （ P〈0.05 ） in formic acid-contained treatments than that in enzymes treated silages. Relative to control, all treatments had lower （P〈0. 05 ） ammonia-N concentrations during ensiling. The enzyme treatments effectively （P〈0. 05 ） decreased neutral detergent fiber and acid detergent fiber contents in the silages. The results suggested that the addition of formic acid and enzymes improved the Lolium multiflorum and Trifolium repens mixture silage quality, and the enzyme treatments were better than formic acid treatments during ensiling.

关 键 词： 纤维素酶 木聚糖酶 甲酸 青贮

领　　域： [农业科学] [农业科学] [农业科学]