作　　者： ; ; ; ; ; ;

机构地区： 北京科技大学

出　　处： 《现代化工》 2013年第2期11-14,16,共5页

摘　　要： 7-氨基头孢烷酸是合成头孢类抗生素的重要中间体,一般由头孢菌素C通过化学法或生物酶法裂解脱去侧链分子制备得到。综述了头孢菌素C酰化酶的来源、特性及其基因的克隆和改造,表达与纯化,固定化及其催化工艺的研究,并展望了一步酶法生产7-氨基头孢烷酸的应用前景。 7-aminocephalosporanic acid, an important intermediate for the preparation of semi-synthetic eephalosporin antibiotics, is generally produced by hydrolysis of cephalosporin C via chemical or enzymatic methods. The research progress in one-step enzymatic conversion of eephalosporin C to 7-aminoeephalosporanic acid, involving research background,origin, properties, gene cloning and evolution of eephalosporin C aeylase, expression and purification of eephalosporin C acylase, enzyme immobilization and catalysis, as well as the application prospects of the novel process, is reviewed. The application prospects of one-step enzymatic conversion of cephalosporin C to 7-aminocephalosporanic acid are out forward as well.

关 键 词： 氨基头孢烷酸 两步酶法 一步酶法 头孢菌素 酰化酶

领　　域： [化学工程]