作　　者： ; ; ; ; (杨晓琳）; (郭刚）;

机构地区： 海南大学环境与植物保护学院

出　　处： 《基因组学与应用生物学》 2013年第1期53-57,共5页

摘　　要： S-层(S-layer)普遍存在于古菌、G^+、G^-菌中,由S-层蛋白所构成细菌S-层结构的生物体中,其功能引起了科学家的广泛关注,但目前S-层的功能大都处于推测阶段。究其原因,是因为外源S-层基因可以造成宿主大肠杆菌的致死效应。本研究将一株苏云金芽胞杆菌的两个S-层蛋白基因(GenBank登录号为AJ012290和AY460125)的3'端序列在大肠杆菌BL21(DE3)中成功进行异源表达,在0.8mmol/LIPTG诱导培养6h时,菌体中包涵体表达量达到最高;并对表达蛋白进行了初步分离纯化,以期为后期利用该纯化蛋白进行抗血清制备,进而对该菌的S-层蛋白在宿主菌中的定位、功能分析打下基础。 Surface layer, S-layer, is a part of the cell envelope commonly found in bacteria （both Gram-positive bacteria and Gram-negative bacteria）, as well as among archaea. The functions of S-layer, attracting wide attention from scientists, are not clearly studied yet due to the lethal effect of exogenous S-layer protein gene to Escherichia coli host strains. Most of the functions we knew currently are deduced and have not been certified. In this study, the 3＇ end sequence from two S-layer protein gene of a Bacillus thuringiensis strain （GenBank accession number AJ012290 and AY460125） have been successfully expressed in Escherichia coli BL21 （DE3）. The expression of the inclusion body in the mycelium reached the highest level after culturing for 6 h in 0.8 mmol/L IPTG. The expressed proteins were preliminary separated and purified, in order to lay a foundation for the preparation of antiserum, and for the localization and function analysis of S-layer protein in the host.

关 键 词： 苏云金芽胞杆菌 层蛋白 异源表达 包涵体

领　　域： [生物学]