作　　者： ; ; ; ; ;

机构地区： 华中农业大学水产学院

出　　处： 《华中农业大学学报》 2013年第2期103-108,共6页

摘　　要： 利用简并引物从日本沼虾肝脏克隆mu型sGST基因cDNA核心片段获得其sGST氨基酸序列。序列分析表明日本沼虾肝脏mu型sGST基因cDNA核心序列长299bp,编码99个氨基酸。日本沼虾sGST与南美白对虾(Litopenaeus vannamei)、小鼠(Mus musculus)、大鼠(Rattus norvegicus)、肩突硬蜱(Ixodes scapu-laris)、扇头蜱(Rhipicephalus appendiculatus)、热带爪蟾(Xenopus tropicalis)、微小牛蜱(Rhipicephalus micro-plus)和太平洋牡蛎(Crassostrea gigas)的mu型sGST基因核苷酸同源性为60%左右,表明所克隆的日本沼虾sGST亦属于mu型sGST。通过对日本沼虾活体浸泡MC-LR,发现微囊藻毒素对日本沼虾肝脏mu型sGST基因表达没有显著的诱导作用。 Soluble glutathione S-transferase（sGST） is extremely important for metabolic detoxication by catalyzing the conjugation of GSH（glutathione） with microcystins.In this study,the amino acid sequence of a mu-class sGST cDNA fragment from the liver of Macrobrachium nipponensis were firstly obtained by RT-PCR using degenerated primers.Sequence analysis revealed that the cloned mu-class sGST cDNA fragment was 299 bp in length,encoding 99 amino acids.Homologous analysis showed that the nucleotide sequence of the glutathione S-transferase gene in M.nipponensis was 60% homologus to the glutathione S-transferase gene of Litopenaeus vannamei,Rattus norvegicus,Mus musculus,Ixodes scapularis,Rhipicephalus appendiculatus,Xenopus tropicalis,Rhipicephalus microplus and Crassostrea gigas.This suggested that the sGST cloned in this study belonged to mu-class GST.The result of further experiment showed that mu-class sGST level did not change significantly in M.nipponensis exposed in MC-LR.

关 键 词： 日本沼虾 谷胱甘肽 转移酶基因 克隆 微囊藻毒素 活体诱导表达

领　　域： [理学]