作　　者： ; ; ; ;

机构地区： 华南农业大学资源环境学院农药与化学生物学教育部重点实验室

出　　处： 《华中农业大学学报》 2013年第2期50-53,共4页

摘　　要： 采用菌丝体生长速率抑制法测定了茶皂素对稻瘟病菌Pyricularia grisea、水稻纹枯病菌Rhizocto-nia solani、番茄小核病菌Sclerotinia sclerotiorum、柑橘青霉病菌Penicillium italicum、黄瓜炭疽病菌Glomer-ella lagenaria、西瓜枯萎病菌Fusarium oxysporum、芒果炭疽病菌Glomerella ciugulata、香蕉炭疽病菌Colle-totrichum musae、黄瓜疫霉病菌Phytophthora parasitica、香蕉枯萎病菌Fusarium oxysporum、玉米小斑病菌Bipolaris maydis、荔枝霜疫霉病菌Peronophythora litchi等12种植物病原菌的抑菌活性。结果表明：以茶皂素质量浓度1 000μg/mL处理后,对稻瘟病菌和柑橘青霉病菌的抑菌率均为100.00%,对黄瓜炭疽病菌、番茄小核病菌、玉米小斑病菌的抑菌率分别为89.12%、87.27%和83.13%;茶皂素质量浓度降至100μg/mL处理后,对稻瘟病菌的抑制率仍为100.00%,表现出良好的生物活性;茶皂素对稻瘟病菌和柑橘青霉病菌的EC50值分别为20.15μg/mL和11.39μg/mL,对香蕉枯萎病菌的EC50超过2 000μg/mL;茶皂素对稻瘟病菌的活性明显优于硫.三环唑,对番茄小核病菌的抑菌活性与速克灵差异不显著。 The inhibition activities of tea saponin against Pyricularia grisea,Rhizoctonia solani,Sclerotinia sclerotiorum,Penicillium italicum,Glomerella lagenaria,Fusarium oxysporumr,Glomerella ciugulata,Colletotrichum musae,Phytophthora parasitica,Fusarium oxysporum,Bipolaris maydis and Peronophythora litchi were studied by mycelium growth rate inhibition assay.The results showed that tea saponin had significant antifungal activity.After treatment with 1 000 μg/mL,the inhibition rates of tea saponin against Pyricularia grisea,Penicillium italicum,Glomerella lagenaria,Sclerotinia sclerotiorum and Bipolaris maydis were 100.00%,100.00%,89.12%,87.27% and 83.13% respectively.The EC50 values against Pyricularia grisea and Penicillium italicum were 20.15 μg/mL and 11.39 μg/mL respectively.Tea saponin had no activity against Fusarium oxysporum with the EC50 value exceeding 2 000 μg/mL.On the other hand,tea saponin had higher antifungal activities against Pyricularia grisea than sulfur tricyclazole,and the inhibition rate against Sclerotinia sclerotiorum was not significantly different betweent tea saponin and procymidone by toxicity comparison in laboratory.

关 键 词： 茶皂素 植物病原菌 抑菌活性

领　　域： [农业科学] [农业科学]