作　　者： ;

机构地区： 中山大学生命科学学院

出　　处： 《中山大学学报（自然科学版）》 2000年第3期78-81,共4页

摘　　要： 吸胀 2 4h的甜菜 (BetavulgarisL cv Loke)种子线粒体通过差速离心和修改的Percoll梯度 (上部为 0 3mol/L甘露醇 ,下部为 0 3mol/L蔗糖 )被纯化 .线粒体位于Percoll梯度的上部 ,大约 30～ 35mL处 .细胞色素C氧化酶 (线粒体的标记酶 )活性、苹果酸脱氢酶活性和蛋白质含量主要分布在此部位 . Mitochondria were purified from sugar beet ( Beta vulgar L cv Loke) seeds which were imbibed for 24 h by differential centrifugation,followed by separation on a modified Percoll gradient containing 0 3 mol/L sucrose in the lower half and 0 3 mol/L mannitol in the upper half Mitochondria band,the activities of cytochrome C oxidase(CCO,a marker enzyme of mitochondria) and malate dehydrogenase(MDH),and protein content were located at the upper fractions(about 30～35 mL) of this type of gradient

关 键 词： 线粒体 纯化 梯度 甜菜 种子

领　　域： [生物学] [农业科学]