作　　者： ; ; ;

机构地区： 中国热带农业科学院热带生物技术研究所农业部热带作物生物技术重点开放实验室

出　　处： 《热带作物学报》 2013年第1期74-80,共7页

摘　　要： 通过对HSM-N培养基的N、P元素含量进行正交试验,得出CE14-M67的最佳油脂积累的N元素浓度为0 mmol/L、P元素浓度为12 mmol/L(即N0P12培养基),此时油脂含量达到70.65%,与HSM-N培养基相比显著上升了12.79%。以N0P12培养基为基础进行葡萄糖、乙酸钠两种碳源的梯度试验。结果显示,诱变株CE14-M67的油脂含量随着葡萄糖浓度的增大而增加,在葡萄糖浓度为10 g/L的培养基中最高达到39.68%,其后,随着葡萄糖浓度的增加,油脂含量有所下降。而在2 g/L乙酸钠的N0P12培养基中,最大油脂含量达69.38%,可见含2 g/L乙酸钠的N0P12培养基是CE-M67的最佳油脂培养基。诱变株CE14-M67在培养8 d时油脂含量达到最大,并且随着pH值的增加油脂含量增大,在pH值为8的培养基中油脂含量达到最大值73%。 A Chlorella vulgaris strain CE14 was isolated from local water area of Hainna Province. By ultraviolet mutagenesis a high oil production algae mutants CE14-M67 was obtained. Further studies showed that the nitrogen-free medium with 12 mmol/L potassium NOP12 medium was the most suitable for oil accumulation for CE14-M67. The oil content of the mutant ceils grown in the medium containing 2 g/L sodium acetate and 10 g/L glucose also could be as high as 69.38% and 39.68%, suggesting that NOP12 contains 2 g/L sodium acetate and 10 g/L glucose was optimum for neutral lipids accumulation. Moreover, the pH value of 8 was optimal for lipid accumulation can reach 73%.

关 键 词： 小球藻 紫外突变 油脂含量 正交实验

领　　域： [生物学]