作　　者： ; ; ; ; ; ;

机构地区： 南京农业大学无锡渔业学院

出　　处： 《上海海洋大学学报》 2013年第1期112-119,共8页

摘　　要： 以酪蛋白和明胶为蛋白源,豆油为脂肪源配制团头鲂幼鱼基础饲料,制成维生素C水平为0.2、33.4、65.8、133.7、251.5mg/kg和501.5 mg/kg的6种等氮等能的试验饲料。每种试验饲料随机投喂3组实验团头鲂幼鱼,每天投喂3次,达饱食水平,试验周期为90 d。结果表明,随着饲料中维生素C添加量的增加,增重率(WG)、特定生长率(SGR)呈先上升而后趋于平缓的趋势,其中251.5 mg/kg和501.5 mg/kg Vc添加组团头鲂WG、SGR显著高于对照组(P<0.05)。各组间肝体比(HSI)和肥满度(CF)无显著性差异,仅133.7 mg/kg Vc添加组团头鲂脏体比(VSI)显著高于对照组(P<0.05)。与对照组相比,饲料中添加65.8～501.5mg/kg Vc显著降低了血液中白细胞(WBC)数量;添加65.8 mg/kgVc显著降低了血清谷草转氨酶(AST)活力,133.7 mg/kg和501.5mg/kg试验组显著降低了谷丙转氨酶(ALT)的水平;添加501.5mg/kg Vc显著提高了团头鲂血清中甘油三酯(TG)的水平;添加133.7 mg/kg Vc显著提高了肌肉中SOD活性(P<0.05)。饲料中维生素C不同添加量对团头鲂幼鱼成活率、血液中红细胞(RBC)数量、血清碱性磷酸酶(ALP)水平、肌肉蒸煮损失等均无显著影响(P>0.05)。以鱼体增重率为评价指标,经折线模型回归分析,得到团头鲂幼鱼饲料中Vc的适宜添加量为150 mg/kg。 A 90-days feeding trial was conducted to estimate the adequate dietary vitamin C（Vc） requirement of juvenile Wuchang bream（Megalobrama amblycephala） and its effect on growth,hematology and muscle physiochemical indexes.450 healthy fish were selected and randomly divided into six groups for which Vc were supplemented at 0.2,33.4,65.8,133.7,251.5 and 501.5 mg/kg diet.After 90 days feeding,the growth performance,hematology and muscle physiochemical indexes were investigated.The results showed that the weight gains（WG） and specific growth rate（SGR） had an increasing trend with the increase of dietary vitamin C,the fish in 251.5 mg/kg and 501.5 mg/kg Vc group had significantly higher SGR that in the control group（P0.05）;while the feed coefficient reduced with increasing of dietary Vc,but no significant differences were observed among dietary treatments（P0.05）.The fish body indices were not significantly different among groups,except the viscerosomatic index in fish with 133.7 mg/kg Vc was significantly higher than that of the control group（P0.05）.Compared with the control,the contents of total leukocytes in fish with 65.8-501.5 mg/kg Vc,serum glutamic-oxaloacetic transaminase in fish with 65.8 mg/kg Vc and serum glutamic-pyruvic transaminase in fish with 133.7 mg/kg and 501.5 mg/kg Vc were significantly reduced（P0.05）.The contents of serum triglycerides in fish with 501.5 mg/kg Vc,superoxide dismutase activities in the muscle in fish with 133.7 mg/kg Vc were significantly improved（P0.05）.Other indices,such as survival rate,erythrocyte,hemoglobin,platelets,albumin and cooking loss of muscle were not significantly different among groups（P0.05）.Based on broken-line model analyses in this study,the minimum dietary vitamin C level is 150 mg/kg to get optimal WG.

关 键 词： 团头鲂 维生素 生长 血液学 肌肉理化指标

领　　域： [农业科学] [农业科学]