作　　者： ; ; ; ; ; ; ; ; ;

机构地区： 广东省农业科学院

出　　处： 《蚕业科学》 2012年第6期981-987,共7页

摘　　要： 桑枯萎病是华南蚕区危害严重的桑树病害。从广东和广西主要蚕区收集桑枯萎病病株样本47份,利用TTC培养基分离到优势菌株105株,根据其培养特性选择10株代表性菌株以水培桑苗进行致病力鉴定,结果表明10个菌株均具有致病性,致病率60%～90%。结合菌株培养特性和16S rRNA序列分析,推断分离选择的10个菌株分别属于肠杆菌科的肠杆菌属(Enterobacter)、克雷白氏杆菌属(Klebsiella)和泛菌属(Pantoea)等3个菌属。基于菌株16S rRNA序列的系统发育分析结果显示:菌株Dafeng08-1和Yingde07属于肠杆菌属的阴沟肠杆菌(E.cloacae),菌株Lianjiang07和Mengshan07属于肠杆菌属的阿氏肠杆菌(E.asburiae),菌株Leizhou07和Wengyuan07属于克雷白氏杆菌属的肺炎杆菌(K.pneumoniae),Xuwen07和Huazhou07菌株属于雷白氏杆菌属的克雷伯氏菌(K.variicola),Guishibie10-1菌株属于克雷白氏杆菌属的产酸克雷伯氏菌(K.oxytoca),Luogang07菌株属于泛菌属的菠萝泛菌(P.ananatis)。由此可见,引起华南蚕区桑枯萎病的病原菌具有多样化特征。 Mulberry wilt disease is one of the mulberry diseases which cause serious damage to mulberry trees in southern sericultural areas of China.In this study,47 samples were collected from mulberry trees infected by mulberry wilt disease in sericultural areas of Guangdong and Guangxi,from which 105 predominant bacterial strains were separated using TTC medium.Based on their cultural characteristics,10 representative strains were selected for further analysis of pathogenicity to mulberry hydroponic seedlings.The results showed that all 10 strains had infectivity to mulberry with infection rate ranging from 60% to 90%.Combined with the cultural characteristics and 16S rRNA sequence analysis,the 10 selected strains were identified as members of the genera Enterobacter,Klebsiella,and Pantoea of Enterobacteriaceae family,respectively.Phylogenetic analysis based on 16S rRNA sequence indicated that strains Dafeng08-1 and Yingde07 belonged to E.cloacae of genus Enterobacter;strains Lianjiang07 and Mengshan07 belonged to E.asburiae of genus Enterobacter;strains Leizhou07 and Wengyuan07 belonged to K.pneumoniae of genus Klebsiella;strains Xuwen07 and Huazhou07 belonged to K.variicola of genus Klebsiella;strain Guishibie10-1 belonged to K.oxytoca of genus Klebsiella;and strain Luogang07 belonged to P.ananatis of genus Pantoea.It is shown that the pathogens causing mulberry wilt disease in southern sericultural areas of China were greatly diversified.

关 键 词： 桑枯萎病 病原菌 致病性 序列 系统发育树

领　　域： [农业科学] [农业科学]