作　　者： ; ; ; ; ;

机构地区： 汕头大学理学院生物系

出　　处： 《化学研究与应用》 2012年第12期1781-1785,共5页

摘　　要： 考察了低分子有机溶剂与无机盐-乙醇/硫酸铵双水相体系萃取螺旋藻多糖的可行性及影响因素。研究结果表明:为除去并有效回收螺旋藻细胞中的蛋白质成分,在藻细胞破碎后进行盐析沉淀蛋白质,再采用传统的热水浸提法,可得到螺旋藻多糖的溶出率为38.44±1.12mg/g干燥粉;通过乙醇/硫酸铵双水相体系的萃取分配,在w(乙醇)=19%,w(硫酸铵)=27.5%(即双水相体系系线长度TLL=42.9),体系相比VR=1.05,pH=7.0时,螺旋藻多糖的收率可达84.5±1.45%,富集因子可达6.2。该研究结果表明廉价的乙醇/硫酸铵双水相萃取螺旋藻多糖将有望开发成为一条简洁、高效、低成本的螺旋藻多糖分离提取工艺。 Extraction of spirulina platensis polysaecharide by ethanoL/（ NH4 ）2SO4 aqueous two-phase system （ATPS）was investigated. To remove and recycle the proteins, salting-out precipitation protein was carried out after spiru]ina platensis cells disruption. Spirulina platensis polysaccharide was extracted by traditional hot water diffusion and the polysaccharide dissolution rate reached 38.44 ± 1.12mg,/g spirulina platensis powder. Spirulina platensis polysaccharide was purified deeply by ethanol/ （ NH＋ ） 2 SO4 ATPS. The yield and enrichment factor of the polysaccharide were 84.5 ± 1.45 % and 6. 2 respectively, on the condition that the tie line length, volume ratio and pH of ATPS were 42. 9,1.05 and 7. 0, respectively. The results indicated that spirulina platensis polysaccharide would be purified by a simple, efficient and low cost extraction technology using inexpensive ethanoL/ （NH4） 2SO4 ATPS.

关 键 词： 双水相 螺旋藻多糖 萃取 系线 相比

领　　域： [理学] [理学]