作　　者： ; ; ; ; ; ;

机构地区： 河北农业大学食品科技学院

出　　处： 《食品科学》 2012年第22期196-199,共4页

摘　　要： 建立高效液相色谱法快速检测血管紧张素转换酶(ACE)抑制活性的方法。色谱条件为:以ODS C18柱(150mm×4.6mm,5μm)为色谱柱,乙睛-水(20:80,V/V,含0.5‰甲酸)为流动相,流速1mL/min,检测波长为228nm。结果表明:马尿酸在0.1～500μg/mL质量浓度范围内线性关系良好,相关系数r=0.9999;平均回收率为89.77%～99.57%,RSD为0.01%～1.52%。不同的ACE抑制剂检测结果证明该方法操作简便快速、重现性好、准确度高,可以作为ACE抑制剂体外活性的检测方法。 A rapid HPLC method was developed for the determination of angiotensin Ⅰ-converting enzyme（ACE） inhibitory activity.Hippuric acid was separated on an ODS C18 column（150 mm × 4.6 mm,5 μm） using a mobile phase consisting of acetonitrile and water（20：80,V/V,containing 0.5‰ methanoic acid） at a flow rate of 1 mL/min and detected at 228 nm.A good linear relationship was observed for hippuric acid over the concentration range of 0.1 to 500 μg/mL with a correlation coefficient of 0.9999.The average spike recovery rates of hippuric acid were 89.77%–99.57% with a relative standard deviation（RSD） of 0.01%–1.52%（n = 3）.The HPLC method was simple,rapid,reproducible,accurate and suitable for the analysis of ACE inhibitory activity in vitro as demonstrated by its applications to determine different ACE inhibitors.

关 键 词： 高效液相色谱 血管紧张素转换酶 马尿酸

领　　域： [轻工技术与工程] [轻工技术与工程]