作　　者： ; ; ; ; ; ; ;

机构地区： 青岛农业大学

出　　处： 《动物医学进展》 2012年第12期138-140,共3页

摘　　要： 为优化空肠弯曲菌分离培养过程并建立PCR鉴定方法,从鸡盲肠内容物中分离培养获得空肠弯曲菌,以该菌特异的保守序列为模板自行设计引物,扩增产物为773bp片段。鸡盲肠内容物分离培养得到空肠弯曲菌,而其他细菌如大肠埃希菌、金黄色葡萄球菌均不能扩增出特异性片段,并对分离培养过程进行了优化。分离培养过程的优化有利于检测工作的进行,而所建立的PCR鉴定方法具有灵敏、特异的特点,可克服传统生化鉴定的缺点,为空肠弯曲菌的检测提供新方法。 To optimiaze the process of isolation culture and establish a rapid detection PCR method of Campylobacter j ejuni. The Campylobacter j ejuni was isolated from the content of chicken cecum,and its specific conservative sequence was used as templates to design primers. The length of amplified product was a fragment with 773 bp. There were no amplified products could be demonstrated in other microorganisms, such as E. coli and Staphylococcus aureus. This PCR assay is sensitive, specific efficient. It can overcome the deficiency of the traditional biochemical identification, and provide new technology for Campylobacter jejunum detection.

关 键 词： 空肠弯曲菌 分离培养 鉴定

领　　域： [农业科学] [农业科学] [农业科学]