作　　者： ; ; ; ; ; ; ; ; ;

机构地区： 南京医科大学基础医学院生物化学与分子生物学系

出　　处： 《南京医科大学学报（自然科学版）》 2012年第11期1483-1487,共5页

摘　　要： 目的:探讨肌醇依赖性激酶1α(inositol requiring enzyme 1α,IRE1α)对非洲爪蟾胰腺发育的影响。方法:通过显微注射基因特异性反义寡核苷酸实现基因敲降;利用整体胚胎原位杂交方法检测基因表达。结果:IRE1α表达于爪蟾发育中的胰腺;利用基因特异性反义寡核苷酸敲降IRE1α后,非洲爪蟾肠道明显异常,胰腺内外分泌标志性基因胰岛素、淀粉酶表达显著减少;胰腺前体细胞的标志基因pdx1和p48表达明显减少;敲降IRE1α下游基因X-盒结合蛋白1(X-box binding protein 1,XBP1)后,非洲爪蟾的胰岛素、淀粉酶表达也明显减少。结论:IRE1α影响非洲爪蟾胰腺发育,可能通过XBP1发挥作用。 Objective：To investigate the effects of inositol requiring enzyme 1α （IRE 1α） on pancreas development of Xenopus laevies. Methods：The gene-specific antisense oligouucleotides,morpholino （MO）s,were microinjected to knockdown IRE 1α and XBP1. The whole mount in situ hybridization of Xenopus embryos was used to detect the gene expression. Results：IRE 1α was expressed in developing pancreas in Xenopus. There were serious defects in gut development observed in IRE 1α knockdown embryos of Xenop us. The expression of the endocrine marker gene （insulin） and the exocrine pancreas marker gene （amylase） was undeteetable during tadpole stages of development in IRE 1α knockdown embryos compared to the control embryos. The expression of the pancreas progenitor cell marker genes （pdxl and p48） was repressed significantly. And after knockdown of XBP1, the expression of insulin and amylase decreased significantly and can＇t be detectable. Conclusion：IRE 1α plays an essential role in pancreas development of Xenopus and may be through XBP1 dependent pathway.

关 键 词： 肌醇依赖性激酶 非洲爪蟾 胰腺发育

领　　域： [生物学]