机构地区: 深圳大学光电工程学院光电子器件与系统教育部重点实验室
出 处: 《光谱学与光谱分析》 2012年第12期3372-3376,共5页
摘 要: 对时间分辨相干反斯托克斯拉曼散射(T-CARS)方法详细地进行理论分析,搭建了时间分辨相干反斯托克斯拉曼散射实验系统,从理论和实验上验证了T-CARS信号的强度与溶液浓度的关系。以无水乙醇和蒸馏水的混合溶液作为样品,测得在波数为2 927cm-1(C—H)处的信号强度随溶液浓度的变化关系。结果表明,当溶液浓度大于35%时,T-CARS信号强度与溶液浓度成平方关系;而当溶液浓度小于20%时,T-CARS信号强度与溶液浓度成线性关系,实验结果与理论分析相吻合。该结论纠正了以前人类对CARS过程的错误认识,有助于人类对CARS过程的进一步了解和应用。而在低浓度时的线性关系表明时间分辨CARS可对低浓度样品尤其是生物样品进行分子识别和线性定量探测,这对分析生物样品和其它低浓度物质具有重要的应用价值。 The authors made a theoretical analysis and experiment research on the relation of time-resolved coherent anti-Stokes Raman scattering (T-CARS) intensity and the sample concentrations in this paper. It was proved experimentally that the T- CARS intensity is quadratic at the concentrationhigher than 35 M, but is linear with the sample concentration at the concentration lower than 20 M, which fits with theoretical analysis. And the research results correct inaccurate previous perceptions, which is conducive to better interpretation and application of the CARS process. The linear relation between the intensity of the CARS with the sample concentration at low concentrations indicates that the CARS is allowed for direct and precise concentration meas- urements, therefore it will be of great importance in biology and biochemistry.
关 键 词: 线性 时间分辨 相干反斯托克斯拉曼散射 浓度