作　　者： ; ; ; ; ; ;

机构地区： 兰州交通大学化学与生物工程学院

出　　处： 《中国食用菌》 2012年第6期40-43,共4页

摘　　要： 对甘南藏族自治州尖顶羊肚菌进行液体发酵培养,以干菌丝体得率为指标,设计正交试验优化其液体发酵培养基和培养条件。实验结果表明最佳培养基组合为白砂糖30 g.L-1、豆粕粉15 g.L-1、KH2PO40.75 g.L-1、MgSO4.7H2O 0.5g.L-1、维生素B10.03 g.L-1。最优液体发酵条件为温度25℃,培养液初始pH7,接种量10 mL,转速120 r.min-1,培养时间8 d。 The medium and liquid fermentation conditions of Morchella conica which growed in Gannan Tibetan au- tonomous prefecture was studied in the paper as the index to dry mycelia yield by orthogonal experiment. The results showed that the best medium components was sugar （30g·L^-1）, soybean powder （15 g·L^-1）, KH2PO4 （0.75 g·L^-1）, Mg- SO4·7H2O （0.5 g·L^-1） and vitamin Bl （0.03 g·L^-1）. The optimal conditions of liquid fermentation were showed as follows： the temperature was 25℃, the initial pH was 7, the strains for inoculation quantity was 10 mL, the rotate speed was 120 r· min^-1 and culture time was 8 days.

关 键 词： 甘南藏族自治州 尖顶羊肚菌 液体发酵

领　　域： [农业科学] [农业科学]