作　　者： ; ; ; ; ; ;

机构地区： 中山大学生命科学学院有害生物控制与资源利用国家重点实验室

出　　处： 《中国水产科学》 2012年第6期915-922,共8页

摘　　要： 对斜带石斑鱼(Epinephelus coioides)卵巢进行组织学分析,根据卵母细胞的发育阶段将卵巢发育划分为7个时期(I-VII),检测斜带石斑鱼不同发育时期卵巢中两种促性腺激素受体(gonadotropin receptors,GtHRs),即卵泡刺激素受体(follicle-stimulating hormone receptor,FSHR)和促黄体素受体(luteinizing hormone receptor,LHR)的mRNA水平,同步分析下游卵巢型芳香化酶基因(Cyp19a)的表达模式。实时荧光定量PCR(Real-time PCR)结果显示,FSHR的mRNA水平在I、II时期的卵巢中较低,在III时期开始上调,在IV、V时期显著升高,在VI时期显著下降。相比之下,LHR的转录水平在卵巢发育早期(I-IV)表达较低,在V时期开始有所升高,在VI时期达到峰值。卵巢内Cyp19a的mRNA在I III时期保持较低水平,在IV、V时期的表达显著增强,在VI时期表达明显减弱。在卵巢发育过程中,Cyp19a的整体表达模式与FSHR较为相似。上述结果表明,斜带石斑鱼FSHR和LHR均参与了卵巢发育的调控,但在不同发育时期功能不同。在卵巢发育过程中,FSHR可能是介导Cyp19a正调控的主要因子。本研究旨为阐明斜带石斑鱼GtH/GtHR信号系统在生殖调控过程中的作用提供基础依据。 The pituitary gonadotropins（GtHs）,follicle-stimulating hormone（FSH） and luteinizing hormone（LH） regulate steroidogenesis and gametogenesis by binding to their respective gonadotropin receptors（FSHR and LHR） in vertebrates.In this research,the ovary development of the orange-spotted grouper（Epinephelus coioides） was first divided into seven phases（phase I to phase VII） according to the histological characteristics of oocytes,and the temporal expression patterns of FSHR and LHR were further detected in parallel with the expression profiles of a downstream factor（Cyp19a） during different developmental phases of the ovary.Quantitative real-time PCR determined the abundance of FSHR was at a relatively low level within phase I and phase II,then elevated gradu-ally in phase III,significantly increased in phase IV and phase V,and remarkably decreased in phase VI.Con-trastingly,the transcripts of LHR remained at a low level during early development of the ovary（phase I–phase IV）,but were stimulated at the beginning of phase V and peaked in phase VI.The transcriptional levels of Cyp19a were very low in the ovary from phase I to phase III,but significantly increased in phase IV and phase V,and then sharply decreased in phase VI.Generally,Cyp19a had a similar expression profile with FSHR.In summary,our results suggest that both FSHR and LHR are involved in the regulation of ovary development,but two receptors may play distinct roles in the different development phases of the ovary.FSHR may be the major activator of Cyp19a transcription.This investigation contributes to elucidate the actions of GtH/GtHR signal systems in re-productive regulation in the orange-spotted grouper.

关 键 词： 斜带石斑鱼 促性腺激素受体 卵巢型芳香化酶基因 卵巢发育

领　　域： [生物学]