作　　者： ; ; ; ; ;

机构地区： 华南农业大学理学院生物材料研究所

出　　处： 《食品工业科技》 2012年第22期323-325,330,共4页

摘　　要： 以竹叶为原料,石油醚与95%乙醇复合溶剂为提取溶剂提取叶绿素,叶绿素提取液直接经过皂化、酸化、铜代、净化和成盐等步骤制备叶绿素铜钠盐。对影响产品得率和质量指标的提取、铜代两个关键工艺过程,利用L9(34)正交实验进行工艺条件优化。结果表明,竹叶叶绿素的最佳提取条件为复合溶剂中95%乙醇体积分数为15%、液固比8:1(v:w)、提取温度55℃、提取时间2h;铜代过程最佳条件为乙醇体积分数85%、加铜量为理论值的2倍、反应温度60℃、pH2.5。叶绿素铜钠盐得率0.38%,检验结果主要指标符合食品添加剂国家标准。 Bamboo leaves were used as raw material,and the composite solvent of petroleum ether and 95% ethanol was used as extraction solvent of chlorophyll.Chlorophyll extracted directly after the step of saponification,acidification,copper replacement,purification,salt formation and so on were prepared as the sodium copper chlorophyllin.There were two key processes,one was the extraction processesaction which affected the product yield and the other was copper replacement processesaction which affected the quality indicators respectively.The process conditions were optimized by L9（34） orthogonal test.The result showed optimum extraction conditions of the bamboo leaves chlorophyll were 15% of the volume fraction of 95% ethanol in the composite solvent,taking the composite solvent eight times the volume of bamboo leaves as extractant,ratio of liquid to solid 8∶1（v∶w）,extraction temperature 55℃,extraction time 2h.Best process conditions of copper replacement were the ethanol volume fraction of 85%,the increase of copper content was two times of the theoretical value,reaction temperature 60℃,pH2.5.The product yield of sodium copper chlorophyllin was 0.38%.Main indicators of the test result were in accordance with the national standards of food additives.

关 键 词： 竹叶 叶绿素 叶绿素铜钠盐 制备工艺 正交实验

领　　域： [轻工技术与工程] [轻工技术与工程]