作　　者： ; ; ;

机构地区： 广州中医药大学中药学院

出　　处： 《食品科技》 2012年第11期286-288,共3页

摘　　要： 采用蒸发光散射检测器(ELSD)测定茶叶下脚料茶梗中茶氨酸的含量。以AE.LichromC18(250mm×4.6mm,5μm)为色谱柱,含0.1%三氟乙酸的乙腈-含0.1%三氟乙酸的水(体积比为2:98)为流动相,流速0.5mL/min,漂移管的温度54℃,载气(N2)1.46×105Pa。茶氨酸在2.55～20.43μg范围内有良好线性关系,平均回收率为98.5%,RSD=2.30%,茶梗中茶氨酸的含量为6.59～17.04mg/g。该方法简单,快速,分离度好,下脚料茶梗仍具有较大的开发利用价值。 To estahlish a method for the determination of the content of in theanine using high-performance liquid chromatography with evaporative light scattering detector （HPLC-ELSD）. HPLC-ELSD analysis was performed on AE. Lichrom C18 （250 mmx4.6 ram, 5 μm）with the mobile phase of 0.1% trifluoroacetic acid acetonitrile solution （A） and 0.1% trifluoroacetie acid water solution （B） （45：55）. Drift tube temperature was 54 %, pressure of nebulizer gas was 1.46x10s Pa. The resolution of theanine was good. The linear calibration curves were obtained over the range of 2.55-20.43 μg. The average recovery of the method was 98.5% with RSD of 2.30%. This method is convenient, accurate and reliable. The tea stalk is still of great re-utilizable value.

关 键 词： 茶梗 茶氨酸 高效液相色谱法 蒸发光散射检测法

领　　域： [理学] [理学]