作　　者： ; ; ; ; ;

机构地区： 华南农业大学林学院

出　　处： 《华南农业大学学报》 2012年第4期519-523,共5页

摘　　要： 采用盆栽法,在干旱胁迫条件下,对华南地区常见树种楝叶吴茱萸Evodia meliaefolia、石斑木Rhaphiolepis in-dica和任豆Zenia insignis叶片中的渗透调节物质(脯氨酸、可溶性糖和可溶性蛋白质)进行了研究.以探明供试树种通过渗透调节来适应干旱胁迫的途径与机理,为华南石漠化干旱区的树种选择提供科学依据.结果表明:在干旱胁迫下,3个树种的脯氨酸和可溶性糖质量比均表现出逐渐增加的趋势,在重度干旱时达到最大值,其中石斑木的质量比最高(脯氨酸为490.86μg/g,可溶性糖为38.13 mg/g),楝叶吴茱萸最低(脯氨酸为278.33μg/g,可溶性糖为27.74 mg/g).而可溶性蛋白质质量比则表现出先增大后减小的规律,其中楝叶吴茱萸和任豆在轻度干旱时先达到最大值,分别为5.97和6.84 mg/g,而石斑木在中度干旱时才达到最大值(7.44 mg/g),重度干旱时,只有石斑木的可溶性蛋白质质量比高出正常水分条件53.64%,表现出很强的渗透调节能力.综上可知:在3个供试树种中,石斑木具有很强的渗透调节能力,对干旱胁迫有较强的适应性,任豆次之,楝叶吴茱萸渗透调节能力最弱,抗旱适应性较差. This study deals with the changes of osmosis-regulating substances （proline, soluble sugar and soluble protein） of three common tree species in south China area, i. e. , Evodia meliaefolia, Rhaphiole- pis indica, and Zenia insignis, under drought stress by means of pot experiment, with an aim to prove the approach and mechanism of how the trial species adapt to the drought stress so as to provide evidence for species selection in south China limestone area. Major findings were as follows： in condition of drought stress, contents of proline and soluble sugar of all three species showed an increasing trend, which peak- ed under heavy drought stress. Among them, R. indica was the highest （proline 490.86 μg/g, soluble sugar 38.13 mg/g）, while E. meliaefolia was the lowest （ proline 278.33 /μg/g, soluble sugar 27.74 mg/g）, Z. insignis was moderate. The content of soluble protein approximated a parabola： first an in- crease, later a decrease. E. meliaefolia（5. 97 mg/g） and Z. insignis（6. 84 mg/g） peaked under light drought stress, while R. indica（7.44 mg/g） peaked in moderate drought stress. In condition of heavy drought stress, only R. indica＇ s content of soluble protein （53.64%） was higher than that of normal condition, which approximated a great osmotic adjustment ability. In conclusion, R. indica shows a great osmotic adjustment ability, which can well adapt to drought stress condition; Z. insignis takes the second place, and E. meliaefolia is the worst.

关 键 词： 干旱胁迫 渗透调节物质 楝叶吴茱萸 石斑木 任豆

领　　域： [农业科学]