作　　者： ; ; ; ; ;

机构地区： 广州医学院第一附属医院

出　　处： 《热带医学杂志》 2012年第9期1080-1082,共3页

摘　　要： 目的通过检测住院患者肠道宏基因组中β-内酰胺酶耐药基因分布情况,分析耐药基因的发生与演化机制。方法提取50例住院患者大便的宏基因组DNA,应用多重PCR及核酸电泳的方法进行检测,统计β-内酰胺酶耐药基因的分布情况。结果 50例患者中共有26例存在β-内酰胺酶耐药基因,包括TEM、SHV、OXA-1、CTX-M-G1、CTX-M-G2、CTX-M-G9、CTX-M-G8/25、LAT/CMY基因等,而VEB、PER、GES、OXA-48、IMP、VIM和KPC基因均未检测到。另外,某些患者的宏基因组DNA样品中还检测到多个耐药基因共同存在。结论住院患者肠道宏基因组中存在多种β-内酰胺酶耐药基因,这些基因可能是临床分离菌株中耐药基因的来源之一。 Objective To analyze the intestinal metagenome in hospitalized patients for the distribution of β-lactamase genes and study the mechanism of transmission and evolution of drug resistance genes.Methods Fifty metagenomic DNA samples were prepared from stools of hospitalized patients.Multiplex PCR and electrophoresis were applied to detect the distribution of β-lactamase genes.Results β-lactamase genes were detected in 26 of the 50 patients,including genes encoding TEM,SHV,OXA-1,CTX-M-G1,CTX-M-G2,CTX-M-G9,CTX-M-G8 /25,and LAT /CMY.The genes encoding VEB,PER,GES,OXA-48,IMP,VIM and KPC were not detected.Furthermore,the presence of multiple genes in the intestinal metagenome was detected in some of the hospitalized patients.Conclusion Multiple β-lactamase genes were present in the intestinal metagenome of hospitalized patients,and these genes may be the source of resistant genes in clinical strains.

关 键 词： 多重 肠道宏基因组 内酰胺酶

领　　域： [生物学]