作　　者： ; ; (冯雪）; (马东洋）; (陈富林）;

机构地区： 兰州大学口腔医学院

出　　处： 《生物医学工程学杂志》 2012年第5期884-888,共5页

摘　　要： 细胞外基质的理化性能影响着细胞的命运并调节组织形成。为探索最佳的软骨细胞体外三维生长环境,本研究通过改变藻酸盐浓度测定了海藻酸盐小球的力学特性及其对软骨细胞增殖能力的影响。凝胶的压缩机械性能通过INSTRON3365测定,结果发现浓度越高其压缩模量越大,表明凝胶对抗应变的能力越强。同时选取4w龄乳兔关节软骨细胞分别包裹在浓度为1%、2%和3%(w/v)海藻酸盐小球中连续培养4周,结果发现所有3组软骨细胞都表现出有限的增殖并形成类似于软骨组织的细胞簇,其中较低浓度组(1%和2%)增殖更明显。这些结果表明软骨细胞在海藻酸盐凝胶小球中连续培养有少量增殖,且较低浓度的藻酸盐更有利于软骨细胞的增殖,说明藻酸盐凝胶的力学特性影响了软骨细胞的增殖能力。 The mechanical properties of natural and synthetic extraeellular matrices affect cellular processes and regu- late tissue formation. In order to explore the optimal environment for chondrocytes growth in vitro ,we investigated the reIationship between the mechanical properties of the alginate beads and the ability of chondrocyte proliferation in this study. We measured the compressive properties of alginate with different concentrations by INSTRON 3365,and found that compressive moduli significantly increased with increasing alginate concentration. The rabbit chondrocytes were encapsulated in 1%, 2% and 3~ （w/v） alginate beads at high （1×107/ml）density. After 4 week＇s culturing, all the three groups resulted in the limited proliferation of the chondrocytes and the formation of cell clusters resemb ling cartilaginous tissues. Chondrocytes proliferation was more rapid on lower concentrate gels（1%, 2 %） than on the higher concentrate gels（3%）. These results suggested that the mechnical properties of scaffold architecture had cer tain effect on chondrocytes proliferation.

关 键 词： 软骨细胞 海藻酸盐 机械性能

领　　域： [生物学]