作　　者： ; ; ; ; ;

机构地区： 河南农业大学生命科学学院

出　　处： 《信阳师范学院学报（自然科学版）》 2012年第4期490-494,共5页

摘　　要： 构建了家蚕50～500 nt非编码RNA的cDNA文库,发现了189个新的ncRNA,其中一个小RNA-Bm-86在家蚕幼虫和蛹中的表达量显著高于卵和成虫.利用生物信息学软件对该ncRNA的特性及其与上下游基因的关系进行了深入分析,并利用Northern杂交和半定量RT-PCR技术对该ncRNA与其宿主基因在家蚕不同组织的表达情况进行了研究.结果发现,该ncRNA属于H/ACA box snoRNA家族,是由家蚕eIF5A基因的第二个内含子转录产生的,且在家蚕中没有明显的靶标位点.分析其上下游基因结构发现,在其上游263 bp处存在着一个可能的启动子位点,表明其有独立转录的倾向.进一步分析Bm-86与其宿主基因eIF5A在家蚕不同组织部位的表达情况发现,二者在表达上存在着负相关的关系,且该现象在丝腺中尤其明显.推测Bm-86可能通过影响eIF5A基因的表达参与到家蚕丝腺发育调控过程中. The cDNA library of 50-500 nt ncRNAs in Bornbyx mori were constructed, and a small RNA Bin-86 accumulated more in larva and pupa during development was found. To further dissect the function of this ncRNA, bioinformatics analysis, northern blot and RT-PCR were taken out. Results showed that Bm-86 was an H/ACA box small nucleolar RNA, located in the second intron of eIF5A, with no predicted pseudouridylation modification site in rRNAs, snRNAs and tRNAs of B. mori. Moreover, a putative promoter were located 263 bp upstream of Bm-86, which indicated that Bm-86 might be transcribed independently. Further analysis showed that the expression of Bin-86 exhibited negative correlation with its host gene eIF5A, notably in silkgland, which indicated that Bin-86 might be involved in the development of silkgland through regulating the expression of eIF5A.

关 键 词： 家蚕 非编码 丝腺

领　　域： [生物学]