作　　者： ; ; ; ; ; ;

机构地区： 中华人民共和国农业部

出　　处： 《中国水产科学》 2012年第5期900-905,共6页

摘　　要： 采用硝酸、双氧水将水产样品中的有机硒氧化成无机硒后测定总硒含量;用50%盐酸提取样品中的无机硒并用硝酸、双氧水消解,测定无机硒含量,再通过差减法获得有机硒的含量。研究结果表明,在优化的工作条件下,硒的检出限为7.1 ng/mL,线性范围为0～20μg/L。经回收实验和实际样品检测,总硒加标回收率为93.88%～107.65%,相对标准偏差(RSD)为0.82%～4.97%;无机硒加标回收率为82.88%～90.10%,RSD为2.33%～7.73%。用标准物质对照,其测定数据完全在标准值范围内。实验表明,该方法线性范围宽,相关性好,精密度和准确度高,可操作性强,应用范围广,适合水产品中有机硒和无机硒测定。 We measured the total selenium content in aquatic product samples after oxidation of organic selenium into inorganic selenium using a nitric acid, perhydrol mixed system. Simultaneously, we measured inorganic selenium content by HG-AFS following extraction of the sample in a HCL（50%） solution and digestion of the water phase in HNO3 and H202. Using these data we calculated the organic selenium content of the samples. Un- der optimal conditions, the detection limit of selenium by this method was 7. 1 ng/mL and the linear range was between 0-20 μg/L. The recovery rate of total selenium was 93.88%-107.65% and the RSD was 0.82%-4.97%. Similarly, the recovery rate of organic selenium was 82.88%-90.10% and the RSD was 2.33%-7.73%. In summary, this method has a wide linear dynamic range and good precision and accuracy, so is suitable for the determination of organic and inorganic selenium in aquatic products.

关 键 词： 原子荧光光谱法 水产品 无机硒 有机硒

领　　域： [农业科学] [农业科学] [环境科学与工程]