作　　者： ; ; ; ; ;

机构地区： 西南大学生命科学学院

出　　处： 《安徽农业科学》 2012年第28期13832-13834,共3页

摘　　要： [目的]研究铬暴露对草鱼(Ctenopharyngodon idellus)的氧化损伤及抗氧化能力的影响。[方法]将草鱼暴露于不同浓度梯度(0、7.23、14.47和28.94 mg/L)的重铬酸钾水体中,于96 h测定肝胰脏中丙二醛(MDA)含量、总抗氧化能力(T-AOC)水平和谷胱甘肽硫转移酶(GST)的活性。[结果]在试验梯度范围内,随着六价铬(Cr6+)浓度的增加,草鱼肝胰脏的MDA含量呈上升趋势;T-AOC水平呈现出先升高后下降的规律性变化,表现为低和中浓度(7.23和14.47 mg/L)下被诱导,而高浓度(28.94 mg/L)下则诱导减弱,其中14.47mg/L组的T-AOC水平显著高于对照组(P<0.05);GST活性除低浓度组(7.23 mg/L)有轻微诱导外,其余各组总体呈下降趋势,其中高浓度组(28.94 mg/L)的GST活性显著低于对照组(P<0.05)。[结论]六价铬可以引起草鱼肝胰脏组织发生更大的氧化损伤而使其出现中毒现象,可能进一步损伤其组织结构和生理功能。 [Objective] This study aimed to investigate the effect of chromium on oxidative damage and antioxidant capacity of grass carp（Ctenopharyngodon idellus）.[Method] The grass carps were treated with hexavalent chromium（Cr6＋） solution at concentrations of 0,7.23,14.47,28.94 mg/L,and then the content of malondialdehyde（MDA）,the level of total antioxidative capacity（T-AOC） and the activity of glutathione-S-transferase（GST） in the hepatopancreas of grass carp were determined after 96 hours in different treatment groups.[Result] The content of MDA presented increasing trend with the increase of exposure Cr6＋ concentrations.The activity of T-AOC increased firstly,then decreased with the increasing Cr6＋ exposure concentrations,showing that the level of T-AOC was induced in low and medium concentrations（7.23 and 14.47 mg/L）,but inhibited in high concentrations（28.94 mg/L）.Among the exposure groups,the level of T-AOC in medium concentration group（14.47 mg/L） was significantly higher than control（P〈0.05）.Except the low concentration groups（7.23 mg/L） of which the GST activity was slightly induced,the GST activities of the other groups all showed downward trend with increasing Cr6＋ levels,and the activity of GST in 28.94 mg/L group was significantly lower than the control group（P〈0.05）.[Conclusion] Cr6＋ could cause large oxidative damage in the hepatopancreas of grass carp,thus poisoning it,and Cr^6＋ may further damage the organizational structure and physiological function of grass carp.

关 键 词： 草鱼 肝胰脏 六价铬 氧化损伤 抗氧化能力

领　　域： [农业科学] [农业科学]