作　　者： ; ; ; ; ;

机构地区： 中国农业科学院北京畜牧兽医研究所动物营养学国家重点实验室

出　　处： 《畜牧兽医学报》 2012年第9期1401-1407,共7页

摘　　要： 通过研究在饲喂小麦-豆粕型日粮和玉米-豆粕型日粮条件下,北京鸭小肠各段肠液组成沿肠道纵向长度的差异,为模拟鸭小肠液的设计提供参考。试验采用完全随机设计,将30只成年北京公鸭随机分配到2个处理中,每个处理5个重复,每个重复3只鸭,分别随机饲喂小麦-豆粕型日粮和玉米-豆粕型日粮,在试验期的第19天屠宰后取十二指肠、空肠前段、空肠后段和回肠的食糜,离心后取上清液分析消化酶活性、离子浓度及pH。研究结果表明,十二指肠肠液与空肠前段肠液的淀粉酶和脂肪酶活性无显著差异(P>0.05),且显著地高于空肠后段肠液和回肠肠液的相应值(P<0.05)。空肠前段肠液与空肠后段肠液的胰蛋白酶活性无显著差异(P>0.05),且显著高于十二指肠肠液和回肠液的相应值(P<0.05)。空肠前段肠液的糜蛋白酶活性最高,其后依次为十二指肠肠液、空肠后段肠液和回肠液,且4种肠液中两两间差异显著(P<0.05)。沿小肠纵向长度,鸭肠液的pH从6.22至7.94逐渐升高,Na+和Ca2+浓度先升后降,K+浓度逐步下降,Mg2+浓度在十二指肠肠液中最低,显著地低于其他肠段肠液的相应值(P<0.05)。日粮组成对肠液中4种消化酶的活性及K+和Mg2+的浓度有显著的影响。由此可见,沿鸭小肠长度的纵向分布中,空肠液的4种主要消化酶活性最高,不同肠段肠液在离子浓度上存在显著差异。 This study was conducted to investigate the difference of intestinal fluid composition along with the intestine of Peking duck fed with wheat-soybean meal diet and corn-soybean meal diet, respectively, which would provide biological basis for simulating intestinal fluid in duck feedstuff evaluation using in vitro digestion. The completely random design was adapted. Thirty adult ducks with similar weight were divided into 2 groups consisting of 5 replicates of 3 birds each and fed ad libitum with wheat-soybean meal and corn-soybean meal diets, respectively. The digesta of duodenum, anterior jejunum, posterior jejunum and ileum were sampled after slaughter at the 19th day of the test period. Intestinal fluid was made by centrifuging digesta samples at 4℃. The activites of digestive enzymes and electrolyte concentration were determined. The re- sults showed that there were higher amylase, lipase activities in duodenal and anterior jejunal flu- id than that in posterior jejunal and ileal fluid （P^0.05）. The activity of trypsin was higher inanterior and posterior jejunal fluid than that in duodenal and ileal fluid （P（0.05）. The anterior jejunal fluid had higher activity of chymotrypsin than that in duodenal, posterior jejunal and ileal fluid （P（0.05）. There was significant difference in chymotrypsin activity between in any two of duodenal, posterior jejunal and ileal fluid. Along with intestine of Peking duck, the pH was in- creased from 6.22 to 7.94. The Na＋ and Ca2＋ concentration were increased and then decreased. The K＋ concentration was slowly decline. The Mg2＋ concentration in duodenal fluid was lower than that in anterior jejunal, posterior jejunal and ileal fluid. Diet treatments significantly affected amylase, lipase, trypsin, and chymotrypsin activities and K＋, Mg2＋ concentration of intestinal fluid. In conclusion, along the duck＇s intestine, jejunal fluid had higher 4 digestive enzyme activ- ities than that in duodenal or ileal fluid, and there were significant difference in t

关 键 词： 北京鸭 小肠液 消化酶 离子浓度

领　　域： [农业科学] [农业科学] [农业科学] [农业科学] [农业科学]