作　　者： ; ; ; ; ; ; ; ; ; ; (滕立典）; (潘捷科）;

机构地区： 南昌大学生命科学与食品工程学院食品科学与技术国家重点实验室

出　　处： 《食品工业科技》 2012年第18期190-194,共5页

摘　　要： 首次从云南红茶中分离筛选到1株高产单宁酶的真菌菌株Aspergillus awamor,其在pH5.0、30℃培养72h后所产胞外单宁酶活力达162.84U/mL。酶活稳定性研究表明,该菌株所产胞外单宁酶在储存4和12周后仍保存98.02%和90.49%的酶活力;所产胞外单宁酶最适酶促反应温度为40℃,且耐高温性能优越,90℃水浴1h仍保留有88%的酶活;pH3.0～6.5范围内酶活均较稳定,酶促反应最适pH5.5;K+、Ag+、Zn2+等金属离子能促进胞外单宁酶酶促反应,而Na+、Mg2+、Ca2+、Fe2+、Fe3+、Co2+、Mn2+、Cu2+、Ba2+等金属离子在不同程度上抑制酶促反应,其中Co2+、Cu2+的抑制作用最强。 One fungal strain named Aspergillus awamori FUYN206,which could produce high yield of tannin acyl hydrolase,was isolated from Yunnan Black Tea.The activity of its extracellular tannase was 162.84U/mL when the strain grown at 30℃,pH5.0 under agitation（120r/min） for 72h.And the activity of extracellular tannase from Aspergillus awamori FUYN206 was reserved 98.02% and 90.49% after stored at 4℃ for four and twelve weeks respectively.The optimal reaction temperature was 40℃.Study on the stability of tannase activity that was shown the extracellular tannase had the superior stability of high-temperature.The activity of this tannase retained around 88% at 90℃ for 1h.The extracellular tannase was stable in the pH range of 3.0～6.5 and showed pH optima of 5.5.The activity of the extracellular tannase was enhanced by K^＋,Ag^＋,Zn^2＋.It was inhibited by Na^＋,Mg^2＋,Ca^2＋,Fe^2＋,Fe^3＋,Hg^2＋,Mn^2＋,Ba^2＋,particularly Co^2＋ and Cu^2＋.

关 键 词： 泡盛曲霉 胞外单宁酶 高温稳定性

领　　域： [轻工技术与工程] [轻工技术与工程]