作　　者： ; ; ; ; ; ; ;

机构地区： 广东出入境检验检疫局

出　　处： 《中国卫生检验杂志》 2012年第8期1739-1741,共3页

摘　　要： 目的:建立食品中黄色2G、奶油黄和柑橘红2含量的高效液相色谱测定方法,采用液质联用法定性。方法:样品中的黄色2G、奶油黄和柑橘红2用正己烷提取,中性氧化铝固相萃取柱净化,采用C18色谱柱(4.6 mm×250 mm,5μm)分离,以甲醇和水溶液为流动相梯度洗脱,二极管阵列检测器检测定量。结果:黄色2G、奶油黄和柑橘红2标准曲线范围为0.5 mg/L～10.0 mg/L,回收率范围为80.3%～95.8%,相对标准偏差为1.51%～5.75%。结论:该法准确度高,分离效果好,结果稳定可靠,适合于食品中黄色2G、奶油黄和柑橘红2含量的测定。 Objective：To develop a method for the quantitative analysis of yellow 2G,butter yellow and citrus Red 2 in foods and identification by liquid chromagraphy mass spectrometry.Methods：The target chemicals in foods were extracted with hexane and purified with neutral alumina solid phase extraction column.The separation was carried out on C18 column（4.6 mm×250 mm,5 μm） at 30℃ with MeOH＋H2O as mobile phase.The flow rate was 1.0 ml.Diode-array detector was used for quantitative analysis.Results：There was good linear relationship in the range of 0.5 mg/L～10.0 mg/L for the three target chemicals.The recovery rate of the method were between 80.3% and 95.8% with RSDs of 1.51%～5.75%.Conclusion：The method is simple,accurate and suitable for the determination of yellow 2G,butter yellow and citrus Red 2 in foods.

关 键 词： 高效液相色谱法 液质联用 黄色 奶油黄 柑橘红 固相萃取

领　　域： [理学] [理学]