作　　者： ; ; ; ;

机构地区： 成都大学

出　　处： 《食品研究与开发》 2012年第7期53-56,共4页

摘　　要： 探讨酶解骨素的最优工艺及对优化实验方法的选择提供借鉴,通过响应面法和正交试验分析了酶解骨素的最优工艺,并对结果进行比较,发现两种试验设计方法在分析各因素对水解度的影响上得出的结果相同,即酶浓度>时间>温度>pH,但是响应面法对数据的统计分析优于正交试验;两种方法获得的最优工艺验证实验显示,响应面法得出的最优工艺所得的水解度比正交试验高出15.4%。酶解骨素的最优工艺确定以响应面法为准,为酶解温度60℃、酶解时间5 h、pH 4、酶用量0.146 mkat/g。 The optimal hydrolysis processing for bone extract was investigated by two methods （orthogonal design experiment and response surface design experiment）; and the obtained optimal hydrolysis processing from the two methods were compared. The results indicated that the two methods obtained the same results when they were used to analyze the effect of single factors on hydrolysis rate, namely, concentration of enzyme 〉time 〉 temperature〉pH, but the response surface method showed a better analysis than that of the orthogonal method. Verification testing results indicated that the hydrolysis rate, from the optimal processing of response surface method, was 15.4 % higher than that of orthogonal method. The optimal processing was decided according to response surface method, and the optimal processing was temperature 60 ℃, time 5 h, pH 4, the addition amount of papaya protease 0.146 mkat/g.

关 键 词： 正交试验 响应面 骨素 酶解

领　　域： [轻工技术与工程] [轻工技术与工程]