机构地区: 广东工业大学轻工化工学院
出 处: 《分析测试学报》 2012年第8期982-986,共5页
摘 要: 合成了二氢杨梅素-锌配合物(DMY-Zn),采用紫外可见光谱、红外光谱、元素分析及热重分析(TG-DTA)法对其结构进行了表征,结果表明二氢杨梅素与Zn2+离子形成了配合物,其组成为[C15H10O8Zn].2H2O。采用EB为荧光探针利用荧光滴定法和粘度法进一步研究了二氢杨梅素-锌配合物与DNA的相互作用,发现二氢杨梅素-锌配合物与DNA有较强的相互作用,能和EB竞争与DNA结合,插入到ctDNA相邻的碱基对中,其作用方式为插入作用,Stern-Volmer线性猝灭常数Ksq为1.01。 A complex of dihydromyricetin-Zn(DMY-Zn)was prepared firstly,the interaction of DMY-Zn complex with DNA was investigated by using fluorescence titration that ethidium bromide(EB) as a fluorescence probe and viscosity measurements.The results indicated that DMY and Zn2+ ions are easy to form complexes which compositions were tested as [C15H10O8Zn]·2H2O by characterization using UV-Vis,IR spectroscopy,elemental analysis and thermal gravimetric analysis(TG-DTA).With the strong interaction with DNA,the DMY-Zn complex can compete with the strong intercalator ethidium bromide to intercalate into the stacked base pairs through the intercalation which the Stern-Volmer quenching constant Ksq is equal to 1.01.