作　　者： ;

机构地区： 仲恺农业工程学院

出　　处： 《安徽农业科学》 2012年第23期11601-11602,共2页

摘　　要： [目的]建立木薯块根RNA的提取方法,并克隆和鉴定淀粉合成酶SSS Ⅱ基因核心片段。[方法]采用改良的CTAB法提取木薯块茎RNA,反转获得cDNA模板,同时基于已知植物的SSS Ⅱ基因同源性,设计简并引物,进行RT-PCR扩增,通过Blast在线检索比对对基因功能进行了初步鉴定。[结果]所获得片段即为木薯SSS Ⅱ基因的核心序列。[结论]该研究为木薯淀粉合成酶SSS Ⅱ基因全长cDNA序列的克隆及其反义载体的构建奠定了基础,同时为实现优质淀粉的代谢工程提供了理想的候选基因。 [Objective] To establish an efficient method to extract RNA from cassava,and clone the core sequence of SSS Ⅱ gene.[Method] The cassava RNA was obtained using the improved CTAB method,which was then reverse transcripted into cDNA.Degenerate primers were designed based on the homology property of known SSS Ⅱ sequences in other plant species.A fragment was amplified with the previously mentioned cDNA as template and the degenerate primers through Polymerase Chain Reaction（PCR）.[Result] After online blasting in NCBI,the sequence was identified to be the core fragment of cassava SSS Ⅱ gene.[Conclusion] The research would lay the original basis for the cloning of the cassava SSS Ⅱ full length cDNA sequence and construction of its anti-sense vector,which could further provide proper candidate genes for the development of starch metabolic engineering.

关 键 词： 木薯 淀粉合成酶 克隆

领　　域： [农业科学] [农业科学]