机构地区: 青岛农业大学
出 处: 《山东农业科学》 2012年第7期22-25,共4页
摘 要: 为了研究茶树对低温胁迫的生理生化反应,揭示茶树适应逆境的生理机制及其与茶树抗寒性关系,本试验以扦插苗福鼎大白和实生苗鸠坑种的两年生枝条为试材,在不同低温胁迫时间下对茶树叶片中超氧阴离子(O2.-)的产生速率以及过氧化氢(H2O2)、丙二醛(MDA)、脯氨酸、可溶性蛋白和可溶性糖的含量进行了测定。结果表明,随着低温胁迫时间的延长,茶树叶片内O2.-产生速率呈现先增高后降低的趋势,H2O2、MDA含量显著升高,脯氨酸含量变化无明显规律;可溶性蛋白和可溶性糖的绝对含量的增加,减缓了细胞膜透性的增加。 In order to study the physiological and biochemical responses of tea trees to low - temperature stress, and reveal the physiological mechanism of stress adaptation and its relationship with the cold resistance of tea tree, the branches of two - year - old Camellia sinensis cv. Fudingdabai and Jiukengzhong were used as materials to determine the generation rate of superoxide anion ( 02 ) and the contents of H202, malondialdehyde ( MDA), proline, soluble protein and soluble sugar in tea leaves under different treat time of cold stress. The results showed that with the prolonging of stress time, the generation rate of O2 increased to a certain degree and then decreased, and the contents of H202 and MDA increased significantly. But the variation of proline content was not regular. The absolute content of soluble protein and soluble sugar increased, which could slow down the increase of cell membrane permeability.