作　　者： ; ; ; ;

机构地区： 韩山师范学院

出　　处： 《植物学报》 2012年第4期422-426,共5页

摘　　要： 以凤凰茶(Camellia sinensis)叶片为例,介绍一种获取高度木质化植物叶片表皮细胞的简易方法——叶片燃烧法。该方法根据高度木质化的叶片在燃烧、过水之后,其上下表皮自行分开、细胞透明化,从而获得材料上下表皮细胞的轮廓。该方法具有简单迅速、制片效率高、上下表皮易区分及可靠稳定等优点,非常适用于木质化叶片表皮特征的研究,也可为植物学及生态学的相关研究方法提供参考。 We have developed an efficient leaf-burning method to observe epidermal cells of tea which are highly lignified in cell walls. With this method, the clear shape of the upper and lower epidermis cells can be observed after several steps of burning and rinsing. After treatment, the upper and lower epidermis cells can automatically separate and hyalinize. This method is easy to use, saves time, distinguishes the upper from the lower side of plant epidermis and has high reliability and stability. The epidermal characteristics of the lignified leaves can be accurately and quickly determined for botany and ecology research.

关 键 词： 燃烧 表皮细胞 凤凰茶 叶片 木质化

领　　域： [生物学]