作　　者： ; ; ; ;

机构地区： 湛江师范学院

出　　处： 《东北林业大学学报》 2012年第7期14-17,44,共5页

摘　　要： 以广州一号桉树优良无性系无菌苗茎段为外植体,通过对噻唑基脲类新型分裂素(N-phenyl-N'-[6-(2-chlorobenzothiazol)-yl]urea,PBU)等多种不同质量浓度植物生长调节剂组合的优化,进行愈伤组织诱导及再生研究。结果表明,在添加了2 mg.L-1PBU和0.06 mg.L-1IAA的改良MS培养基上,茎段外植体5 d后愈伤组织诱导率达100%;将愈伤组织接种在添加1 mg.L-16-BA和0.1 mg.L-1NAA的MS培养基上,诱芽率高达90%;随后,在添加了0.2 mg.L-1PBU与0.05 mg.L-1NAA的1/2MS培养基上诱导芽伸长,用1/2MS培养基附加0.2 mg.L-1IBA诱导生根,炼苗15 d后,以质量m(黄心土)∶m(腐殖质土)=1∶1为基质进行移栽,得到完整再生植株,成活率高达95%。 The callus induction and shoot regeneration of elite clone of Eucalyptus Guangzhou No. 1 were studied systematically using a variety of combinations of different plant growth regulators, such as N-phenyl-N＇- [ 6- （2-chlorobenzothiazol）-yl ] urea （PBU）. Sterile clones of seedling stem segments were used as explants and cultured in a modified MS medium supplemen- ted with the synthetic growth regulator 2mg · L^-1 PBU and 0.06mg · L^-1 IAA. After cultivation for 5 days, 100% of explants formed callus. Calluses were transferred to MS medium containing 1 mg · L^-1 6-BA and 0.1 mg · L^-1 NAA to induce bud formation, and 90% of the callus induced by PBU exhibited adventitious bud formation. Shoot elongation was then stimulated on 1/2MS supplemented with 0.2mg · L^-1 PBU and 0.05mg · L^-1 NAA for 20 days. For rooting, the elongated shoots were cultivated on root induction medium containing 0.2 mg · L^-1 IBA. After 15 days of acclimatization, the survival rate of seedlings reached 95% with a mixture of yellow soil and humus soil （ 1 ： 1 by volume） as a substrate for transplanting, and fully mature plants were obtained.

关 键 词： 广州一号桉树 愈伤组织诱导 再生

领　　域： [农业科学] [农业科学]