作　　者： ; ; ; ; ; ;

机构地区： 泸州医学院附属医院

出　　处： 《中华麻醉学杂志》 2012年第5期559-562,共4页

摘　　要： 目的探讨肠缺血再灌注对大鼠脑组织小胶质细胞活化的影响。方法清洁级健康成年雄性sD大鼠128只，体重250—300g，采用随机数字表法，将其随机分为2组（n=64）：假手术组（S组）和肠缺血再灌注组（I／R组）。I／R组采用夹闭肠系膜上动脉90min后再灌注的方法制备肠缺血再灌注损伤模型。于再灌注2、6、24、48h时观察肠粘膜病理学结果，并行Chiu评分；取脑组织，计数活化的小胶质细胞，计算小胶质细胞活化率，测定脑组织活性氧（ROS）、MDA含量及SOD活性、NO含量、一氧化氮合酶（NOS）及诱导型一氧化氮合酶（iNOS）活性。结果与S组比较，I／R组肠组织Chiu评分、脑组织活化的小胶质细胞数、小胶质细胞活化率、ROS、NOS和iNOS活性、MDA和NO含量升高，SOD活性降低（P〈0．05或0．01）；I／R组再灌注6～48h脑组织ROS、NOS和iNOS活性、MDA和NO含量依次升高，脑组织SOD活性及肠组织Chiu评分依次降低，脑组织活化的小胶质细胞和小胶质细胞活化率于再灌注24h时达峰值（P〈0．05或0．01）。结论肠缺血再灌注可通过激活脑组织小胶质细胞，激活NOS和促进ROS生成，从而诱发脂质过氧化反应，该作用作为肠缺血再灌注诱发大鼠脑损伤的机制。 Objective To investigate the effects of intestinal ischemia-reperfusion （I/R） on cerebral microglial activation in rats. Methods One hundred and twenty-eight healthy male SD rats weighing 250-300 g were randomly allocated to one of two groups （ n = 64 each） ： group sham operation （group S） and intestinal I/R group. Intestinal I/R was produced by occlusion of superior mesenteric artery for 90 min followed by reperfusion. Sixteen animals were sacrificed at each of the 4 time points ： 2, 6, 24 and 48 h of reperfusion in each group. Their intestines were obtained for microscopic examination. Their brains were harvested for detection of microglial activation （by immuno-histochemistry）. The reactive oxygen species （ROS）, MDA and NO contents and SOD, nitric oxide synthase （NOS） and inducible nitric oxide synthase （iNOS） activities in the brain were measured. Results The microglia were in quiescent condition. Ibal staining was negative or light in group S. Intestinal I/R significantly increased intestinal Chiu score, cerebral microglial activation at 6,24 and 48 h of reperfusion which peaked at 24 h of reperfusion in group I/R as compared with group S. Cerebral ROS, MDA, NO levels and NOS, iNOS activities were significantly higher while SOD activity was significantly lower in group I/R than in group S. Conclusion Intestinal I/R can activate microglia and induce the release of nitrogen and oxygen free radicals resulting in cerebral injury.

关 键 词： 肠 再灌注损伤 小神经胶质细胞 脑

领　　域： [生物学]